This antibody is expected to recognize both human isoforms.
纯化方法
IRF5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
IRF5 antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of IRF5.
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 57 kDa band observed in A549 lysate . In transfected HEK293 transiently expressing IRF5 a strong band of approximately 60 kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 57.8 kDa according to NP_001092099.1. Recommended concentration, 0.1-0.3 μg/mL. IHC: This antibody showed nuclear staining in Human Cervix, as confirmed by anonymous customer (data not shown). Chromatin immunoprecipitation: This antibody has been successfully used in human monocyte-derived dendritic cells.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.