KEAP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
KEAP1 antibody was raised against a amino acid synthetic peptide near the internal region (near N-Terminus) of KEAP1 (aa 41-53).
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 75 kDa band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 69.7 kDa according to human NP_036421.2 and of 69.6 kDa according to mouse NP_057888.1). Recommended concentration: 0.2-0.6 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
KEAP1
(Kelch-Like ECH-Associated Protein 1 (KEAP1))