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- 抗原 See all HRAS 抗体
- HRAS (HRas proto-oncogene, GTPase (HRAS))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This HRAS antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- The antibody H-Ras-03 reacts with human H-Ras, an ubiquitously expressed 21 kDa intracellular protein. Although reactivity with other species has not been determined, it is probable as the epitope is highly conserved among animals.
- 产品特性
- Synonyms: GTPase HRas, HRAS1, p21ras, H-Ras-1, c-H-ras, Ha-Ras, H-Ras, v-Ha-ras Harvey rat sarcomaviral oncogene homolog
- 纯化方法
- Protein A affinity chromatography.
- 免疫原
- Peptide corresponding to amino acids DIHQYREQIKRVKDSDDC of human H-Ras protein.
- 克隆位点
- H-Ras-03
- 亚型
- IgG1
- Top Product
- Discover our top product HRAS Primary Antibody
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anti-HRas proto-oncogene, GTPase (HRAS) (C-Term) antibody
HRAS 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-HRas proto-oncogene, GTPase (HRAS) antibodyHRAS 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-HRas proto-oncogene, GTPase (HRAS) antibodyHRAS 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 1C2 unconjugated
anti-HRas proto-oncogene, GTPase (HRAS) (AA 104-128) antibodyHRAS 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-HRas proto-oncogene, GTPase (HRAS) antibodyHRAS 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A1 unconjugated
anti-HRas proto-oncogene, GTPase (HRAS) (AA 104-128) antibodyHRAS 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB15622 unconjugated
anti-HRas proto-oncogene, GTPase (HRAS) (AA 146-176) antibodyHRAS 适用: 人, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-HRas proto-oncogene, GTPase (HRAS) (AA 2-186) antibodyHRAS 适用: 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-HRas proto-oncogene, GTPase (HRAS) (AA 12-181) antibodyHRAS 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-HRas proto-oncogene, GTPase (HRAS) antibodyHRAS 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA. Western Blotting under Reducing conditions (0.5-1 μg/mL): Positive control: RAJI human Burkitt lymphoma cell lineHeLa human cervix carcinoma cell lineK567 human leukemia cell lineSample preparation: Resuspend approx.50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith reducing Laemmli SDS-PAGE sample buffer. Boil for 5 min.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH ~7.4, 15 mM Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid freeze/thaw cycles.
Shelf life: One year from despatch. - 有效期
- 12 months
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- 抗原
- HRAS (HRas proto-oncogene, GTPase (HRAS))
- 别名
- HRAS (HRAS 产品)
- 别名
- C-BAS/HAS antibody, C-H-RAS antibody, C-HA-RAS1 antibody, CTLO antibody, H-RASIDX antibody, HAMSV antibody, HRAS1 antibody, K-RAS antibody, N-RAS antibody, RASH1 antibody, hras antibody, zgc:110250 antibody, HRAS antibody, H-RAS antibody, c-H-ras antibody, H-Ras antibody, K-Ras antibody, hras1 antibody, rash1 antibody, ras antibody, N-Ras antibody, c-bas/has antibody, H-ras antibody, Ha-ras antibody, Harvey-ras antibody, Hras-1 antibody, Kras2 antibody, c-Ha-ras antibody, c-rasHa antibody, hrasl antibody, zgc:110734 antibody, HRas proto-oncogene, GTPase antibody, v-Ha-ras Harvey rat sarcoma viral oncogene homolog a antibody, neuroblastoma RAS viral (v-ras) oncogene homolog pseudogene antibody, Harvey rat sarcoma viral oncogene homolog L homeolog antibody, Harvey rat sarcoma viral oncogene homolog antibody, Harvey rat sarcoma virus oncogene antibody, NRAS proto-oncogene, GTPase antibody, -Ha-ras Harvey rat sarcoma viral oncogene homolog b antibody, HRAS antibody, hrasa antibody, LOC733587 antibody, Hras antibody, hras.L antibody, hras antibody, NRAS antibody, hrasb antibody
- 背景
- H-Ras is one of three ubiquitously isoforms of Ras GTPase that operate at the intracellular leaflet of the plasma membrane to regulate multiple signal transduction pathways, such as mitogen-activated protein kinase (MAPK) cascade. H Ras is anchored to the plasma membrane by farnesyl and two palmityl residues. GTP loading decreases H-Ras affinity for lipid rafts and allows the protein to target to nonraft microdomains, the primary sites of H-Ras signaling. Sos protein and other guanine nucleotide-exchange factors catalyze dissociation of GDP from Ras. Besides its roles in the plasma membrane, active H-Ras also diffuses through the cytoplasm on nanoparticles termed rasosomes, which is dependent on Ras palmitoylation.Synonyms: GTPase HRas, H-Ras, H-Ras-1, HRAS1, Ha-Ras, c-H-ras, p21ras, v-Ha-ras Harvey rat sarcoma viral oncogene homolog
- 基因ID
- 3265
- UniProt
- P01112
- 途径
- p53 Pathway, MAPK Pathway, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling, BCR Signaling
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