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Mesothelin 抗体 (Extracellular, Extracellular Domain)

Name: Mesothelin 抗体 (Extracellular, Extracellular Domain)
Brand: antibodies-online
SKU: ABIN233752
Availability: OutOfStock

MSLN 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal MB-G10 unconjugated

Immunohistochemistry using anti-mesothelin antibodies to detect mesothelin in PEFF human tissue sections treated by antigen retrieval methods. (Mesothelin 抗体 (Extracellular, Extracellular Domain))

IHC

SDS-PAGE of Mouse anti-Mesothelin Monoclonal Antibody. (Mesothelin 抗体 (Extracellular, Extracellular Domain))

SDS

Western blotting using anti-mesothelin antibodies to detect mesothelin-Fc at 100 ng (lane 1), 25 ng (lane 2), 6 ng (lane 3), 2 ng (lane 4) and 0. (Mesothelin 抗体 (Extracellular, Extracellular Domain))

3 images

(2 references)

产品编号 ABIN233752

发货至: 中国

Datasheet as PDF

抗原 See all Mesothelin (MSLN) 抗体

Mesothelin (MSLN)
抗原表位
16

9

6

6

5

5

5

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
Extracellular, Extracellular Domain
适用
89

24

5

2

2

2

1

1

1
人
宿主
76

24

4

1

1
小鼠
Compatible Secondaries
克隆类型
74

30

2
单克隆
标记
68

12

8

6

1

1

1

1

1

1

1

1

1

1

1

1
This Mesothelin antibody is un-conjugated
应用范围
67

51

24

21

12

11

9

5

4

4

2

2

2

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC)

特异性

This antibody is directed against human mesothelin protein. This product was purified from tissue culture supernatant fluid by Protein A chromatography. Cross reactivity with homologues from other sources has not been tested.

产品特性

Mesothelin is a glycosyl-phosphatidyl- inositol-anchored glycoprotein present on the cell surface of various human solid tumors. The mesothelin (MSLN) gene encodes a 71-kDa precursor protein that is processed to a 40-kDa glycosylphosphatidyl-inositol-anchored protein that composes the mature portion and an NH₂-terminal 31-kDa fragment called megakaryocyte-potentiating factor that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers. The biological functions of mesothelin remain elusive. A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.

过滤

Sterile filtered

免疫原

This antibody was produced in mice by repeated immunizations with a recombinant protein corresponding to the extracellular domain of human mesothelin.
Immunogen Type: RecombinantProtein

克隆位点

MB-G10

亚型

IgG2a

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MSLN 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

应用备注

This antibody has been tested for use in immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kDa in size corresponding to mature mesothelin by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, archival PEFF human tissues were deparaffinized followed by hydration. Antigen-retrieval is recommended. Block tissues with 1% BSA in PBS for 30 min at 23° C. Antibodies are diluted in 1% BSA and reacted with tissue for 60 min at room temperature.

说明

Gene Name: MSLN

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C/-20 °C

储存方法

Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.

有效期

3 months
Ho, Bera, Willingham, Onda, Hassan, FitzGerald, Pastan: "Mesothelin expression in human lung cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 13, Issue 5, pp. 1571-5, (2007) (PubMed).

Onda, Willingham, Nagata, Bera, Beers, Ho, Hassan, Kreitman, Pastan: "New monoclonal antibodies to mesothelin useful for immunohistochemistry, fluorescence-activated cell sorting, Western blotting, and ELISA." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 11, Issue 16, pp. 5840-6, (2005) (PubMed).
抗原

Mesothelin (MSLN)

别名

Mesothelin (MSLN 产品)

别名

MPF antibody, SMRP antibody, MSLN antibody, mesothelin antibody, MSLN antibody, Msln antibody, LOC611363 antibody, LOC100524016 antibody

背景

Mesothelin is a glycosyl-phosphatidyl- inositol–anchored glycoprotein present on the cell surface of various human solid tumors. The mesothelin (MSLN) gene encodes a 71-kDa precursor protein that is processed to a 40-kDa glycosylphosphatidyl-inositol–anchored protein that composes the mature portion and an NH₂-terminal 31-kDa fragment called megakaryocyte-potentiating factor that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers. The biological functions of mesothelin remain elusive. A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.
Synonyms: Mesothelian, MN, MB, Pre-pro-megakaryocyte-potentiating factor, CAK1 antigen

基因ID

10232, 53988378

UniProt

Q13421

途径

EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process