HRAS 抗体 (AA 62-76)
Quick Overview for HRAS 抗体 (AA 62-76) (ABIN224929)
抗原
See all HRAS 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 62-76
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特异性
- Recognizes phosphorylated and non-phosphorylated human, rat and mouse ras. Does not cross-react with the rap protein. Neutralizes the biological and biochemical activities by binding to residues E63, S65, A66, M67, Q70 and R73. Positive Controls: SW480 or Y1 cells (IF and IP), normal skin (IHC, frozen and paraffin), A431, Hela or ML-1 cells (WB).
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纯化方法
- Purified
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免疫原
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Recombinant p21 ras protein. Epitope: aa62-76 of human ras.
Type of Immunogen: Recombinant protein -
亚型
- IgG1 kappa
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anti-HRas proto-oncogene, GTPase (HRAS) (C-Term) antibody
HRAS 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-HRas proto-oncogene, GTPase (HRAS) antibodyHRAS 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 1C2 unconjugated
anti-HRas proto-oncogene, GTPase (HRAS) (AA 104-128) antibodyHRAS 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-HRas proto-oncogene, GTPase (HRAS) antibodyHRAS 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A1 unconjugated
anti-HRas proto-oncogene, GTPase (HRAS) (AA 104-128) antibodyHRAS 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB15622 unconjugated
anti-HRas proto-oncogene, GTPase (HRAS) (AA 146-176) antibodyHRAS 适用: 人, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-HRas proto-oncogene, GTPase (HRAS) (AA 2-186) antibodyHRAS 适用: 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-HRas proto-oncogene, GTPase (HRAS) (AA 12-181) antibodyHRAS 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-HRas proto-oncogene, GTPase (HRAS) antibodyHRAS 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 小鼠 Monoclonal H-Ras-03 unconjugated
anti-HRas proto-oncogene, GTPase (HRAS) (AA 1-189) antibodyHRAS 适用: 人 WB, PLA 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Approved: IF (2.5 μg/mL), IHC, IHC-Fr, IHC-P, IP, Neut, WB (2.5 μg/mL)
Usage: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Neutralization. Immunofluorescence: 2.5 μg/mL. Western Blot (chemiluminescence detection): 2.5 μg/mL. Immunohistochemistry (Frozen): 5 μg/mL. Immunohistochemistry: 1-5 μg/mL. Staining of formalin fixed, paraffin embedded tissue requires pepsin pretreatment. Use saponin to permeablize. Immunoprecipitation (autoradiographic detection): 1 μg/sample SW480 or Y1 cells. Use rabbit anti-rat IgG plus Protein A or goat anti-rat IgG plus Protein G. Protein A alone will not immunoprecipitate rat IgG. Doublets corresponding to farnesylated- and non-farnesylated ras may be observed. Detects both phosphorylated and non-phosphorylated human ras as well as the p21 translational products of the H-, K- and N-ras human genes. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4, 0.1 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- HRAS (HRas proto-oncogene, GTPase (HRAS))
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别名
- HRAS / H-Ras
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背景
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Name/Gene ID: HRAS
Subfamily: RAS oncogene
Family: Ras GTPase superfamily IPR001806
Synonyms: HRAS, C-H-RAS, C-has/bas p21 protein, C-ras-Ki-2 activated oncogene, C-HA-RAS1, CTLO, H-ras, H-RASIDX, GTP- and GDP-binding peptide B, GTPase HRas, H-Ras-1, HAMSV, HRAS1, K-RAS, Ha-Ras, Ha-Ras1 proto-oncoprotein, Transforming protein p21, N-ras, p21ras, RASH1, C-BAS/HAS, C-ha-ras, p19 H-RasIDX protein -
基因ID
- 3265
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UniProt
- P01112
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途径
- p53 Pathway, MAPK Pathway, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling, BCR Signaling
抗原
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