ERK2 抗体 (C-Term)
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Quick Overview for ERK2 抗体 (C-Term) (ABIN221147)
抗原
See all ERK2 (MAPK1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- GST fusion protein. The epitope maps near the carboxy-terminus of human Erk-2. The epitope is identical to the corresponding sequence in mouse and differs from the rat sequence by a single, conservative amino acid substitution.
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免疫原
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Fusion protein from human MAPK1 / ERK.
Type of Immunogen: Fusion protein
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应用备注
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Approved: ELISA, WB
Usage: This antiserum was tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 42 kD in size corresponding to p42 MAP Kinase (ERK2) by western blotting in the appropriate cell lysate or extract. p42 MAP Kinase (ERK2) is a ubiquitous protein kinase target for Ras and Raf. The following cell lines have been assayed by immunoblot and were found to be positive for p42 MAP Kinase (ERK2) using this reagent: U937, HeLa, NIH-3T3, RAW 264.7, LNCaP and HEK whole cell lysates. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
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储存条件
- -20 °C
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储存方法
- Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid freeze-thaw cycles. Centrifuge product if not completely clear after standing at room temperature.
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- ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))
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别名
- MAPK1 / ERK2 / p42
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背景
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Name/Gene ID: MAPK1
Subfamily: MAPK
Family: Protein Kinase
Synonyms: MAPK1, ERK, ERK-2, ERK2, MAP kinase 1, MAPK 2, MAPK2, MAP kinase isoform p42, MAPK 1, p41mapk, PRKM2, MAP kinase 2, p41, p42-MAPK, PRKM1, ERT1, p38, p40, p42MAPK, Protein tyrosine kinase ERK2 -
基因ID
- 5594
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UniProt
- P28482
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途径
- MAPK Pathway, RTK signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins
抗原
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