-
- 抗原 See all CD4 抗体
- CD4
-
适用
- 小鼠
-
宿主
- 大鼠
-
克隆类型
- 单克隆
-
标记
- This CD4 antibody is conjugated to APC
-
应用范围
- Flow Cytometry (FACS)
- 产品特性
- The 4AGK1.5 monoclonal antibody reacts with the mouse CD4 Molecule, a 55 kDa cell surface receptor. It is a member of the lg superfamily,primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC classII and associating with the protein tyrosine kinase, lck.
- 亚型
- IgG2b
- Top Product
- Discover our top product CD4 Primary Antibody
-
anti-CD4 (CD4) (AA 385-457) antibody
CD4 适用: 人, 小鼠, 大鼠, 小鸡, Pig FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD4 (CD4) antibodyCD4 适用: 人, 小鼠, 大鼠, 小鸡, 猴, Pig, 犬, Cat, 兔, Cow, 绵羊, 豚鼠, 山羊, 马, Avian, 驴, Hamster WB 宿主: 山羊 Polyclonal unconjugated
anti-CD4 (CD4) (AA 231-330) antibodyCD4 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD4 (CD4) (N-Term) antibodyCD4 适用: 人 FACS, WB, CyTOF 宿主: 小鼠 Monoclonal MEM-241 unconjugated
anti-CD4 (CD4) (AA 300-400) antibodyCD4 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-CD4 (CD4) (AA 301-400) antibodyCD4 适用: 人, 小鼠, 大鼠 FACS, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD4 (CD4) antibodyCD4 适用: 小鼠 FACS, IP, IHC (fro), ICC, Func 宿主: 大鼠 Monoclonal GK1-5 unconjugated
anti-CD4 (CD4) (N-Term) antibody (FITC)CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 FITC
anti-CD4 (CD4) (N-Term) antibody (PE)CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 PE
anti-CD4 (CD4) antibody (PE)CD4 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal OX-35 PE
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
-
- Take 100 μL a certain amount of prepared cells and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.3-0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined in two hours on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
-
- 抗原
- CD4
- 别名
- CD4 (CD4 产品)
- 别名
- cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody
- 途径
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
-
You are here:
