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- 抗原 See all CD45RO products
- CD45RO
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD45RO antibody is conjugated to PE-Cy5
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应用范围
- Flow Cytometry (FACS)
- 产品特性
- 4AUCHL1 reacts with an isoform of CD45, a 180 kDa molecule clustered as CD45RO.
- 克隆位点
- 4AUCAL-1
- 亚型
- IgG2a
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anti-CD45RO (CD45RO) antibody
CD45RO 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD45RO (CD45RO) antibodyGMP Grade CD45RO 适用: 人 FACS, IHC, IP 宿主: 小鼠 Monoclonal UCHL1 unconjugated
anti-CD45RO (CD45RO) antibody (FITC)GMP Grade CD45RO 适用: 人 FACS 宿主: 小鼠 Monoclonal UCHL1 FITC
anti-CD45RO (CD45RO) antibody (PE)GMP Grade CD45RO 适用: 人 FACS 宿主: 小鼠 Monoclonal UCHL1 PE
anti-CD45RO (CD45RO) antibody (mFluor™450)GMP Grade CD45RO 适用: 人 FACS 宿主: 小鼠 Monoclonal UCHL1 mFluor™450
anti-CD45RO (CD45RO) antibody (APC)GMP Grade CD45RO 适用: 人 FACS 宿主: 小鼠 Monoclonal UCHL1 APC
anti-CD45RO (CD45RO) antibody (PE-CF®594)GMP Grade CD45RO 适用: 人 FACS 宿主: 小鼠 Monoclonal UCHL1 PE-CF®594
anti-CD45RO (CD45RO) antibodyCD45RO 适用: 人, Chimpanzee, Marmoset FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal UCHL-1 unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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- 抗原
- CD45RO
- 别名
- CD45RO (CD45RO 产品)
- 背景
- CD45RO is expressed on memory/activated T cells and cortical thymocytes, in comparison with CD45RA, which is expressed on naive/resting T cells and on medullart thymocytes. CD45RO and CD45RA molecules are important to discriminate naive and memory T cells in the study of the immune system.
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