CD45RO 抗体
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- 抗原 See all CD45RO products
- CD45RO
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适用
- 人, Chimpanzee, Marmoset
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD45RO antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 产品特性
- This antibody recognizes a 180-185 kDa protein, identified as isoform of leukocyte common antigen (CD45) called CD45RO (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. CD45RO is found on mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. The CD45RO antibody shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, antibody to CD45RO is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Cultured human T-cells from an IL-2-dependent T-cell line (CA1) were used as the immunogen for the CD45RO antibody.
- 克隆位点
- UCHL-1
- 亚型
- IgG2a kappa
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anti-CD45RO (CD45RO) antibody
CD45RO 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD45RO (CD45RO) antibodyGMP Grade CD45RO 适用: 人 FACS, IHC, IP 宿主: 小鼠 Monoclonal UCHL1 unconjugated
anti-CD45RO (CD45RO) antibody (FITC)GMP Grade CD45RO 适用: 人 FACS 宿主: 小鼠 Monoclonal UCHL1 FITC
anti-CD45RO (CD45RO) antibody (PE)GMP Grade CD45RO 适用: 人 FACS 宿主: 小鼠 Monoclonal UCHL1 PE
anti-CD45RO (CD45RO) antibody (mFluor™450)GMP Grade CD45RO 适用: 人 FACS 宿主: 小鼠 Monoclonal UCHL1 mFluor™450
anti-CD45RO (CD45RO) antibody (APC)GMP Grade CD45RO 适用: 人 FACS 宿主: 小鼠 Monoclonal UCHL1 APC
anti-CD45RO (CD45RO) antibody (PE-CF®594)GMP Grade CD45RO 适用: 人 FACS 宿主: 小鼠 Monoclonal UCHL1 PE-CF®594
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- 应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD45RO antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the CD45RO antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- CD45RO
- 别名
- CD45RO (CD45RO 产品)
- 背景
- This antibody recognizes a 180-185 kDa protein, identified as isoform of leukocyte common antigen (CD45) called CD45RO (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. CD45RO is found on mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. The CD45RO antibody shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, antibody to CD45RO is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
- 基因ID
- 5788
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