Nestin 抗体
Quick Overview for Nestin 抗体 (ABIN201334)
抗原
See all Nestin (NES) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes rodent Nestin at 200-220kD. Specificity confirmed with mouse and rat. Does not cross-react with human.
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纯化方法
- Purified
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免疫原
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Nestin purified from embryonic rat spinal cord.
Type of Immunogen: Purified protein -
亚型
- IgG1
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anti-Nestin (NES) (AA 178-399) antibody
NES 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal D10 unconjugated
anti-Nestin (NES) antibodyNES 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 小鼠 Monoclonal 2C2F7 unconjugated
anti-Nestin (NES) (AA 252-290), (N-Term) antibodyNES 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 1389-1416) antibodyNES 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB16253 unconjugated
anti-Nestin (NES) antibodyNES 适用: 小鼠 IHC, ICC 宿主: 大鼠 Monoclonal 7A3 unconjugated
anti-Nestin (NES) (AA 698-844) antibodyNES 适用: 人 IHC (f) 宿主: 小鼠 Monoclonal NES-2911 unconjugated
anti-Nestin (NES) (AA 1484-1500) antibodyNES 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 1389-1416) antibodyNES 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 681-750) antibodyNES 适用: 人, 小鼠, 大鼠 ELISA, FACS, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ICC (1:20 - 1:200), IHC, IHC-Fr (1:10 - 1:100), WB
Usage: Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Immunohistochemistry (frozen or paraffin): 1:20-1:200. Perfuse tissue with 4 % paraformaldehyde, pH 7.4 for light microscopy or with either 4 % paraformaldehyde, pH 10 or 4 % paraformaldehyde with 0.1 % glutaraldehyde, pH 7.4 for EM. Immunocytochemistry: 1:20-1:200. Fix cells in 4 % paraformaldehyde buffered with 50 mM sodium borate, pH 9.5. Western Blot: 200-220kD in reducing gels of newborn rat or mouse cell extracts. Prepare samples by boiling in 4 volumes of 125 mM Tris, pH 6.8, 10 % 2-mercaptoethanol, 10 % glycerol, 4.6 % SDS. 5 % PAGE gels are suggested. Block membranes with milk or BSA. Do not dilute primary antibody in buffer containing milk. Freezing and thawing of samples or samples not stored at -70?C will show breakdown of nestin sometimes into multiple fragments. Positive control: Mouse embryonic brain tissue.
Not recommended for: IHC-P -
说明
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Target Species of Antibody: Rat
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Tris-glycine, 0.15 M sodium chloride, pH 7.4, 0.05 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Nestin (NES)
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别名
- Nestin
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背景
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Name/Gene ID: NES
Synonyms: NES, Nestin, Nbla00170 -
基因ID
- 10763
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UniProt
- P48681
抗原
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