P44/42 MAP Kinase antibody detects endogenous levels of total p44/42 MAP Kinase protein.
纯化方法
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
免疫原
The antiserum was produced against synthesized non-phosphopeptide derived from human p44/42 MAP Kinase around the phosphorylation site of tyrosine 204 (T-E-YP-V-A).
Western Blot: 1: 500approx. 1: 1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
抗原
ERK1/2 (MAPK1/3)
(Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))
erk1/2 antibody, mitogen-activated protein kinase antibody, erk1/2 antibody
背景
Both p44 and p42 MAP kinases (Erk1 and Erk2) function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1] or 183 and 185 of rat Erk2) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). Both kinases are known to weakly autophosphorylate on tyrosine.Synonyms: ERK-1/ERK-2, Extracellular signal regulated kinase 1/2, Insulin stimulated MAP2 kinase, MAPK1/MAPK2, Microtubule associated protein 2 kinase, Mitogen activated protein kinase 1/2/3, P42/P44-MAPK