ERK1/2 抗体 (AA 300-380)
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Quick Overview for ERK1/2 抗体 (AA 300-380) (ABIN7206459)
抗原
See all ERK1/2 (MAPK1/3) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 300-380
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原理
- Rabbit Anti-ERK 1/2 Polyclonal Antibody
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特异性
- ERK 1/2 Polyclonal Antibody detects endogenous levels of ERK 1/2 protein.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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免疫原
- Synthesized peptide derived from the C-terminal region of human ERK 1/2 at AA range: 300-380
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亚型
- IgG
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:50-200,WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,Not yet tested in other applications,
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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有效期
- 12 months
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- ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))
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别名
- ERK 1/2
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背景
- MAPK3, ERK1, PRKM3, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubule-associated protein 2 kinase, pMitogen-activated protein kinase 3 encoded by MAPK3 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.
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分子量
- 42,44kD
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基因ID
- 5595
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UniProt
- P27361, P28482
抗原
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