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ERK1/2 抗体 (pTyr205, pTyr222)

Name: ERK1/2 抗体 (pTyr205, pTyr222)
Brand: antibodies-online
SKU: ABIN7206465
Availability: OutOfStock

The 兔多克隆 anti-ERK1/2 antibody is suitable to detect ERK1/2 in samples from 人, 小鼠和大鼠. It has been validated for WB, IHC, ELISA 和 IF.

Western Blot analysis of KB(1), MCF-7(2), diluted at 1:500. (ERK1/2 抗体 (pTyr205, pTyr222))

Immunohistochemical analysis of paraffin-embedded rat lung tissue. (ERK1/2 抗体 (pTyr205, pTyr222))

IHC

Immunohistochemical analysis of paraffin-embedded human lung tissue. (ERK1/2 抗体 (pTyr205, pTyr222))

IHC

Immunohistochemical analysis of paraffin-embedded mouse liver tissue. (ERK1/2 抗体 (pTyr205, pTyr222))

IHC

4 images

产品编号 ABIN7206465

发货至: 中国

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中国
北京 101111

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Beijing Economic Technological Development Area

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Quick Overview for ERK1/2 抗体 (pTyr205, pTyr222) (ABIN7206465)

抗原

See all ERK1/2 (MAPK1/3) 抗体

ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This ERK1/2 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Discover our top product MAPK1/3 Primary Antibody

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pTyr205, pTyr222

原理

Rabbit Anti-ERK1/2 (phospho Tyr222/205) Polyclonal Antibody

特异性

Phospho-ERK 1/2 (Y222/205) Polyclonal Antibody detects endogenous levels of ERK 1/2 protein only when phosphorylated at Y222/205.

纯化方法

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

免疫原

Synthesized peptide derived from human ERK1/2 Phospho-Tyr222/205

亚型

IgG
anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (AA 301-358) antibody
MAPK1/3 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IHC (p), IF (cc), FACS, IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) antibody
MAPK1/3 适用: 人, 小鼠, 大鼠, Cow, Pig, 斑马鱼, 犬, 鱼, 绵羊, 猴, 马 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (AA 300-380) antibody
MAPK1/3 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ChIP 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (pThr202), (pTyr204) antibody
MAPK1/3 适用: 人, 小鼠, 大鼠, Cow WB, ELISA, IHC (p), IF (cc), FACS, IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (pThr183), (pTyr185) antibody
MAPK1/3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (C-Term) antibody
MAPK1/3 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) antibody
MAPK1/3 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 3F8 unconjugated

anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (pThr183), (pTyr185) antibody
MAPK1/3 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, IHC (p), IF (cc), FACS, IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) antibody
KD Validated MAPK1/3 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB4510 unconjugated Recombinant Antibody

anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) (pThr202), (pTyr204) antibody
MAPK1/3 适用: 人, 小鼠 WB, ELISA, DB 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:50-200,WB 1:500-2000,IHC 1:50-300,

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

有效期

12 months
抗原

ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

别名

ERK1/2

背景

MAPK1, ERK2, PRKM1, PRKM2, Mitogen-activated protein kinase 1, MAP kinase 1, MAPK 1, ERT1, Extracellular signal-regulated kinase 2, ERK-2, MAP kinase isoform p42, p42-MAPK, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2, MAPK3, ERMAPK1 encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this MAPK1.

分子量

44kD

基因ID

5594, 5595

UniProt

P28482, P27361