(5 references)-
- 抗原
- TAP Tag
- 适用
- 请咨询
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), ELISA
- 序列
- CSSGALDYDI PTTASENLYF Q
- 特异性
- Rabbit Anti-TAP-tag Polyclonal Antibody specifically reacts with fusion proteins containing TAP epitope tag.
- 产品特性
- Rabbit Anti-TAP-tag Polyclonal Antibody is generated against the common tag, which is composed of a 21-residue peptide, CSSGALDYDIPTTASENLYFQ, derived from the C-terminus of the TAP-tag construct after TEV cleavage.
- 纯化方法
- Immunoaffinity chromatography
- 免疫原
- Peptide CSSGALDYDIPTTASENLYFQ conjugated to KLH
- 亚型
- IgG
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anti-TAP Tag antibody
WB 宿主: 小鼠 Monoclonal Mix unconjugated
anti-TAP Tag antibody (HRP)WB, ELISA 宿主: 兔 Polyclonal HRP
anti-TAP Tag antibodyWB, IP 宿主: 小鼠 Monoclonal unconjugated
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- 应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0. 05-0. 2 µg/mL
Western blot: 0. 1-1. 0 µg/mL
Western blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit L00204, C is recommended. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.
Immunoprecipitation (IP):2-10 µg/mg of lysate
Other applications: user-optimized - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.
- 浓度
- 0.5 mg/mL
- 缓冲液
- lyophilized with PBS, pH 7.4, containing 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
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: "Cdc28 kinase activity regulates the basal transcription machinery at a subset of genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 26, pp. 10450-5, (2012) (PubMed).
: "Rrn7 protein, an RNA polymerase I transcription factor, is required for RNA polymerase II-dependent transcription directed by core promoters with a HomolD box sequence." in: The Journal of biological chemistry, Vol. 286, Issue 30, pp. 26480-6, (2011) (PubMed).
: "Kinetic analysis reveals the ordered coupling of translation termination and ribosome recycling in yeast." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 51, pp. E1392-8, (2011) (PubMed).
: "The Saccharomyces cerevisiae anaphase-promoting complex interacts with multiple histone-modifying enzymes to regulate cell cycle progression." in: Eukaryotic cell, Vol. 9, Issue 10, pp. 1418-31, (2010) (PubMed).
: "Floxin, a resource for genetically engineering mouse ESCs." in: Nature methods, Vol. 7, Issue 1, pp. 50-2, (2009) (PubMed).
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: "Cdc28 kinase activity regulates the basal transcription machinery at a subset of genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 26, pp. 10450-5, (2012) (PubMed).
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- 抗原
- TAP Tag
- 物质类
- Tag
- 背景
- Well-characterized antibodies against short-sequence epitope tags are common in the study of protein expression in several different expression systems. The TAP (tandem affinity purification)-tag allows rapid purification of complexes from a relatively small number of cells even if the researcher does not have prior knowledge of the complexs composition, activity, or function. Combined with mass spectrometry, the TAP-tag strategy enables the identification of proteins interacting with a given target protein1
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