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- 抗原
- TAP Tag
- 适用
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- HRP
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应用范围
- Western Blotting (WB), ELISA
- 序列
- CSSGALDYDI PTTASENLYF Q
- 特异性
- Rabbit Anti-TAP-tag [HRP] Polyclonal Antibody is specific to fusion proteins containing TAP epitope tag.
- 纯化方法
- Immunoaffinity chromatography
- 免疫原
- Synthetic peptide CSSGALDYDIPTTASENLYFQ conjugated - KLH.
- 亚型
- IgG1
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anti-TAP Tag antibody
WB 宿主: 小鼠 Monoclonal Mix unconjugated
anti-TAP Tag antibodyWB, IP 宿主: 小鼠 Monoclonal unconjugated
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- 应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL.Western blot: 0.5-1 µg/mLOther Applications: user-optimized - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4, containing 1 % BSA and 0.01 % thimerosal
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
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- 抗原
- TAP Tag
- 别名
- TAP-tag
- 物质类
- Tag
- 背景
- Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.The TAP (tandem affinity purification) tag allows rapid purification of complexes from a relatively small number of cells without prior knowledge about the composition, activity, or function of the complex. Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The Rabbit anti-TAP tag consists a 21 residue peptide, CSSGALDYDIPTTASENLYFQ, derived from c-terminal of the TAP construct after TEV cleavage.Rabbit Anti-TAP-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. The antibody is suitable for detecting fusion proteins that contain TAP tag.
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