TAP 标记 抗体 (HRP)
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- 抗原
- TAP Tag
- 适用
- 标记
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- HRP
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应用范围
- Western Blotting (WB), ELISA
- 交叉反应
- 标记
- 交叉反应 （详细）
- TAP-Tag
- 纯化方法
- Purified by Protein A.
- 免疫原
- Recombinant TAP-Tag
- 克隆位点
- Mix-mA
- 亚型
- IgG
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anti-TAP Tag antibody
WB 宿主: 小鼠 Monoclonal Mix unconjugated
anti-TAP Tag antibody (HRP)WB, ELISA 宿主: 兔 Polyclonal HRP
anti-TAP Tag antibodyWB, IP 宿主: 小鼠 Monoclonal unconjugated
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- 应用备注
- WB 1:300-5000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- TAP Tag
- 别名
- TAP-Tag
- 物质类
- Tag
- 背景
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Synonyms: TAP Tag, TAPTag, Tandem affinity purification
Background: Tandem affinity purification (TAP) is a purification technique for studying proteinprotein interactions. It involves creating a fusion protein with a designed piece, the TAP tag, on the end. The original TAP method involves the fusion of the TAP tag to the C-terminus of the protein under study. The TAP tag consists of calmodulin binding peptide (CBP) from the N-terminal, followed by tobacco etch virus protease (TEV protease) cleavage site and Protein A, which binds tightly to IgG. The relative order of the modules of the tag is important because Protein A needs to be at the extreme end of the fusion protein so that the entire complex can be retrieved using an IgG matrix.
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