CD4 抗体 (Non-Polymorphic Site)
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- 抗原 See all CD4 抗体
- CD4
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抗原表位
- Non-Polymorphic Site
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This CD4 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS)
- 特异性
- SM019LE recognises Murine CD4, a 55 kDa cell surface antigen expressed by a subset of T lymphocytes. This Low Endotoxin CD4 antibody is reported to block MHC class II dependant T-cell responses in vitro and in vivo and induces tolerance.
- 交叉反应 (详细)
- Species reactivity (tested):Mouse.
- 纯化方法
- Ammonium Sulphate Precipitation.
- 内毒素水平
- Low Endotoxin
- 免疫原
- Mouse spleen cells. Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
- 克隆位点
- YTS177-9
- 亚型
- IgG2a
- Top Product
- Discover our top product CD4 Primary Antibody
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- 应用备注
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Flow Cytometry: 1/50 - 1/200, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.4 without preservatives.
- 储存液
- Without preservative
- 注意事项
- This product is photosensitive and should be protected from light
- 储存条件
- 4 °C
- 储存方法
- Store the antibody at 2 - 8 °C. DO NOT FREEZE!
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- 抗原
- CD4
- 别名
- CD4 (CD4 产品)
- 别名
- cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody
- 背景
- CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin Intracellular ligands: p56Lck CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus, CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
- 基因ID
- 12504
- NCBI登录号
- NP_038516
- UniProt
- P06332
- 途径
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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