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IL-6 抗体

IL6 适用: 人 WB, IF, IHC (p), EIA, Func 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN181728
发货至: 中国
  • 抗原 See all IL-6 (IL6) 抗体
    IL-6 (IL6) (Interleukin 6 (IL6))
    适用
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    克隆类型
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    多克隆
    标记
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    This IL-6 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)
    纯化方法
    Affinity Chromatography employing immobilized hIL-6 matrix
    免疫原
    Highly pure (>98%) E.coli derived recombinant Human Interleukin 6.
    Top Product
    Discover our top product IL6 Primary Antibody
  • 应用备注
    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhIL-6 (1.0 ng/mL), a concentration of 0.20 - 0.40 μg/mL of this antibody is required. ELISA: Indirect: To detect hIL-6 (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-6. Sandwich: To detect hIL-6 (using 100 μL/well antibody solution) a concentration of0.5 - 2.0 μg/mL of this antibody is required. In conjunction with Biotinylated Anti-HumanIL-6 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hIL-6. Western blot: To detect hIL-6 this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hIL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing
    限制
    仅限研究用
  • 溶解方式
    Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
    缓冲液
    PBS, pH 7.2 without preservatives
    储存液
    Without preservative
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原
    IL-6 (IL6) (Interleukin 6 (IL6))
    别名
    Interleukin-6 (IL6) (IL6 产品)
    别名
    BSF2 antibody, HGF antibody, HSF antibody, IFNB2 antibody, IL-6 antibody, Il-6 antibody, ILg6 antibody, Ifnb2 antibody, il6 antibody, CHIL-6 antibody, interleukin 6 antibody, interleukin-6 antibody, IL6 antibody, Il6 antibody, il-6 antibody, IL-6 antibody
    背景
    Interleukin 6 (IL6) is a multifunctional 24 kDa protein originally discovered in the medium of RNA stimulated fibroblastoid cells. It is upregulated by IL1, TNF, PDGF, IFN beta, TNF alpha, NGF, IL17 and downregulated by glucocorticoids IL4, TGF beta. IL6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important as IL1 and TNF alpha in regulating the acute phase response. IL6 has also been implicated in regulating adipose mass. IL6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. In addition, IL6 appears to interact with IL2 in the proliferation of T lymphocytes. IL6 potentiates the proliferative effect of IL3 on multipotential hematopoietic progenitors.Synonyms: B-cell stimulatory factor 2, BSF-2, CDF, CTL differentiation factor, Hybridoma growth factor, IFNB2, IL-6, Interferon beta-2
    基因ID
    9606
    UniProt
    P05231
    途径
    TLR signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome
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