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CD25 抗体

IL2RA 适用: 人 WB, IHC (fro), Func, EIA 宿主: 小鼠 Monoclonal unconjugated
产品编号 ABIN181143
发货至: 中国
  • 抗原 See all CD25 (IL2RA) 抗体
    CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
    适用
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    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This CD25 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Enzyme Immunoassay (EIA)
    特异性
    This antibody detects Human CD25.
    产品特性
    Synonyms: Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TACantigen
    纯化方法
    Affinity Chromatography on Protein G
    免疫原
    Highly pure (>98%) recombinant Human soluble IL-2 Receptor alpha
    亚型
    IgG2a
    Top Product
    Discover our top product IL2RA Primary Antibody
  • 应用备注
    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman sIL-2RA (10.0 μg/mL), a concentration of 1.0-1.5 μg/mL of this antibody is required,in the presence of 0.5 ng/mL recombinant hIL-2. ELISA: In a Sandwich ELISA (assuming 100μL/well), a concentration of 4.0-8.0 μg/mL of thisantibody will detect at least 100 pg/mL of recombinant Human sIL-2RA when used inconjunction with compatible secondary reagents. Western Blot: To detect Human sIL-2RA by Western Blot analysis this antibody can be usedat a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human sIL-2RA is 2.0-4.0 ng/lane, underreducing or non-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Restore in sterile water to a concentration of 1.0 mg/mL.
    缓冲液
    PBS without preservatives
    储存液
    Without preservative
    储存条件
    4 °C/-20 °C
    储存方法
    The lyophilized antibody is stable at room temperature for one month or at -20 °C forlonger. Following reconstitution it is stable at 2 - 8 °C for six weeks. Avoid repeated freezing and thawing.
    Shelf life: one year from despatch.
    有效期
    12 months
  • 抗原
    CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
    别名
    CD25 / IL2RA (IL2RA 产品)
    别名
    CD25 antibody, IDDM10 antibody, IL2R antibody, TCGFR antibody, IL2RAC antibody, IL-2RA antibody, Il2r antibody, Ly-43 antibody, interleukin 2 receptor subunit alpha antibody, interleukin-2 receptor subunit alpha-like antibody, interleukin 2 receptor, alpha chain antibody, IL2RA antibody, Il2ra antibody, LOC100222957 antibody
    背景
    The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55
    基因ID
    3559
    UniProt
    P01589
    途径
    JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
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