CD25 抗体 (AA 201-268)
(1 validation)-
- 抗原 See all CD25 (IL2RA) 抗体
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
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抗原表位
- AA 201-268
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CD25 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 交叉反应
- 人, 小鼠
- 预测反应
- Rat
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic peptide derived from mouse CD25/IL2RA
- 亚型
- IgG
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anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody
IL2RA 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 1B5D12 unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) antibodyIL2RA 适用: 人 WB, ELISA, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibodyIL2RA 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) antibodyIL2RA 适用: 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-245) antibodyIL2RA 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 42-183) antibodyIL2RA 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal IL2RA-2395 unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) antibodyIL2RA 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-181 unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (PerCP)IL2RA 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-181 PerCP
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-235) antibodyIL2RA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-185) antibodyIL2RA 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400 - 限制
- 仅限研究用
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- by
- TRON
- No.
- #104539
- 日期
- 2024.02.28
- 抗原
- CD25
- Lot Number
- AG10271265
- Method validated
- Immunohistochemistry
- Positive Control
FFPE human tonsil
- Negative Control
- Notes
Passed. The CD25 antibody ABIN670371 recognizes CD25 in human tonsil tissue.
- Primary Antibody
- ABIN670371
- Secondary Antibody
- HRP-conjugated goat anti-rabbit antibody (medac diagnostics, DPVR-110HRP, Lot 101122)
- Full Protocol
- Dewax and rehydrate tissue sections on glass slides in a descending xylene and graded alcohol series:
- xylene 2x for 5 min.
- 100 % (v/v) ethanol 2x for 5 min.
- 96 % (v/v) ethanol 2x for 5 min.
- 70 % (v/v) ethanol 2x for 5 min.
- Wash slides 2x 5 min with ddH2O.
- Cook slides for 30 min at 95 °C in citrate buffer pH 6.
- Wash slides 2x 3 min with ddH2O.
- Incubate slides in 0.3 % (v/v) H2O2 in PBS for 15 min to quench endogenous peroxidase.
- Wash slides 3x 3 min in 1X PBS.
- Block slides in 10 % goat serum in PBS for 30 min at RT.
- Incubate slides with primary rabbit anti-CD25 antibody (antibodies-online, ABIN670371, lot AG10271265) diluted 1:250 and 1:500 in 10 % goat serum in PBS ON at 4 °C in a humidified chamber.
- Wash slides 3x 3 min in 1X PBS.
- Incubate slides with secondary HRP-conjugated goat anti-rabbit antibody (medac diagnostics, DPVR-110HRP, Lot 101122) ready-to-use in 10 % goat serum in PBS for 30 min at RT in a humidified chamber.
- Wash slides 3x 3 min in 1X PBS.
- HRP development.
- Counterstain slides with hematoxylin solution (Carl Roth, T865.2).
- Wash slides for 15 min under running tap water.
- Dehydrate sections in an ascending alcohol-xylene series:
- 70% ethanol 2x for 10 sec.
- 96% ethanol 2x for 10 sec.
- 100% ethanol 2x for 10 sec.
- xylene 2x for 10 sec.
- Mount sections with Entellan new mounting medium (Sigma-Aldrich, 1008690500).
- Dry sections.
- Image acquisition on an Aperio CS2 Digital Pathology Slide Scanner (Leica), Magnification 40x.
- Experimental Notes
ABIN670371 appears to successfully recognize CD25 in human tonsils, but in mouse spleen and lung, the signal is not present where expected.
ABIN670371 does not appear to recognize CD25 in mouse samples. CD25 should be highly expressed on Tregs, which by definition express FoxP3. ABIN670371 signal does not co-localize with FoxP3 in either spleen or lung. Furthermore, in both tissues, the signal from ABIN670371 is nuclear, whereas it is expected to be membrane. ABIN670371 signal does not co-localize with CD8/CD4 in either spleen or lung although CD25 should (at least partially) co-localize with CD8 and CD4.
生效 #104539 (Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![IIHC staining of human tonsil with anti-CD25 antibody ABIN670371 (1:250) and counterstain with hematoxylin.]()
IIHC staining of human tonsil with anti-CD25 antibody ABIN670371 (1:250) and counterstain with hematoxylin.
![IHC staining of human tonsil with anti-CD25 antibody ABIN670371 (1:500) and counterstain with hematoxylin.]()
IHC staining of human tonsil with anti-CD25 antibody ABIN670371 (1:500) and counterstain with hematoxylin.
Full Methods -
- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- 有效期
- 12 months
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- 抗原
- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
- 别名
- IL2RA (IL2RA 产品)
- 别名
- CD25 antibody, IDDM10 antibody, IL2R antibody, TCGFR antibody, IL2RAC antibody, IL-2RA antibody, Il2r antibody, Ly-43 antibody, interleukin 2 receptor subunit alpha antibody, interleukin-2 receptor subunit alpha-like antibody, interleukin 2 receptor, alpha chain antibody, IL2RA antibody, Il2ra antibody, LOC100222957 antibody
- 背景
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Synonyms: CD25, Il2r, Ly-43, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, p55, Il2ra
Background: The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009].
- 基因ID
- 16184
- UniProt
- P01590
- 途径
- JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
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