Nestin 抗体 (FITC)
(1 validation)Quick Overview for Nestin 抗体 (FITC) (ABIN1774766)
抗原
See all Nestin (NES) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody recognises nestin, a member of the intermediate filament family required for brain and eye development.
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纯化方法
- Protein G purified
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免疫原
- A 150-amino-acid fragment from the cloned human nestin conjugated to GST
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亚型
- IgG1
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anti-Nestin (NES) (AA 178-399) antibody
NES 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal D10 unconjugated
anti-Nestin (NES) antibodyNES 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 小鼠 Monoclonal 2C2F7 unconjugated
anti-Nestin (NES) (AA 252-290), (N-Term) antibodyNES 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 1389-1416) antibodyNES 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB16253 unconjugated
anti-Nestin (NES) antibodyNES 适用: 小鼠 IHC, ICC 宿主: 大鼠 Monoclonal 7A3 unconjugated
anti-Nestin (NES) (AA 698-844) antibodyNES 适用: 人 IHC (f) 宿主: 小鼠 Monoclonal NES-2911 unconjugated
anti-Nestin (NES) (AA 1484-1500) antibodyNES 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 1389-1416) antibodyNES 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 681-750) antibodyNES 适用: 人, 小鼠, 大鼠 ELISA, FACS, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Nestin (NES) (AA 1392-1621) antibodyNES 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal antibody dilution should be determined by titration.
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限制
- 仅限研究用
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- by
- Ensigna Biosystems
- No.
- #029824
- 日期
- 2014.11.06
- 抗原
- Lot Number
- I030314
- Method validated
- Immunofluorescence
- Positive Control
- PC-3 cells
- Negative Control
- MCF-7 cells
- Notes
- Faint fluorescent signal was detected in the positive control cells. No signal was detected in the negative control cells or isotype negative control.
- Primary Antibody
- Antigen: Nestin (NES) (FITC)
- Catalog number: ABIN1774766
- Lot number: I030314
- Dilution: 1:75, 1:100
- Secondary Antibody
- Full Protocol
- Prostate and breast adenocarcinoma cell lines were grown directly on chamber slides, washed with 1X PBS, and fixed with 4% paraformaldehyde in 1X PBS for 15 min at room temperature (RT).
- Fixed cells were rinsed three times in PBS for 5 min each at RT.
- Cells were blocked in 1X PBS / 5% normal goat serum / 0.3% Triton X-100 for 60 min at RT.
- Cells were rinsed two times in 1X PBS.
- Cells were incubated with primary antibody diluted 1:75 and 1:100 in 1X PBS / 1% BSA / 0.3% Triton X-100 overnight at 4°C in dark conditions.
- Cells were rinsed three times in PBS for 5 min each at RT.
- Coverslips were mounted on slides with DAPI-Fluoromount-G.
- Stained cells were imaged with a Nikon Eclipse E600 microscope and Olympus DP70 camera.
- Experimental Notes
- The anti-Nestin antibody showed positive staining of weak intensity localized appropriately to the cell cytoplasm in a pattern consistent with intermediate filaments in positive control PC3 cells. No staining was seen in the negative control MCF7 cells or with the isotype control antibody. Although specificity of the antibody was acceptable, its sensitivity with the current protocol was questionable. It is, however, entirely possible that if this antibody is used with an appropriate amplification protocol the sensitivity would be increased.
生效 #029824 (Immunofluorescence)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Legend: Immunofluorescence. Nestin (green fluorescence) is present in positive control cells (PC3) and absent in negative control cells( MCF7). Scale bar = 50 μm, Magnification = 20X, FITC Exposure = 1.5s, Insets at 40X magnification.
Details:
Left panel: Micrograph image of PC3 Nestin-positive prostate adenocarcinoma cells (top) and MCF7 Nestin-negative breast adenocarcinoma cells (bottom) with 15 μg/mL of anti-Nestin-FITC Ab.
Middle panel: Micrograph image of PC3 Nestin-positive prostate adenocarcinoma cells (top) and MCF7 Nestin-negative breast adenocarcinoma cells (bottom) with 10 μg/mL of anti-Nestin-FITC Ab.
Right panel: Micrograph image of reagent isotype control FITC-conjugated mouse IgG1 antibody on PC3 Nestin-positive prostate adenocarcinoma cells (top) and MCF7 breast adenocarcinoma cells (bottom).]()
Legend: Immunofluorescence. Nestin (green fluorescence) is present in positive control cells (PC3) and absent in negative control cells( MCF7). Scale bar = 50 μm, Magnification = 20X, FITC Exposure = 1.5s, Insets at 40X magnification.
Details:
Left panel: Micrograph image of PC3 Nestin-positive prostate adenocarcinoma cells (top) and MCF7 Nestin-negative breast adenocarcinoma cells (bottom) with 15 μg/mL of anti-Nestin-FITC Ab.
Middle panel: Micrograph image of PC3 Nestin-positive prostate adenocarcinoma cells (top) and MCF7 Nestin-negative breast adenocarcinoma cells (bottom) with 10 μg/mL of anti-Nestin-FITC Ab.
Right panel: Micrograph image of reagent isotype control FITC-conjugated mouse IgG1 antibody on PC3 Nestin-positive prostate adenocarcinoma cells (top) and MCF7 breast adenocarcinoma cells (bottom).
Full Methods -
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Purified IgG conjugated to Fluoroscein using Innova Biosciences Lightning-Link®, supplied in Phosphate buffered saline (PBS) containing 0.09 % Sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Nestin (NES)
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别名
- Nestin
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背景
- Neural Stem Cell Marker
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基因ID
- 407242
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OMIM
- 600915
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UniProt
- P18175
抗原
-
