Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
特异性
Epitopes for Species: H:Y302,M:Y300,R:Y300
纯化方法
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
免疫原
The antiserum was produced against synthesized phosphopeptide derived from human A-RAF around the phosphorylation site of tyrosine 301/tyrosine 302 (G-Y-YP-W-E).