AKT1 抗体 (pSer473)
(1 reference)-
- 抗原 See all AKT1 抗体
- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
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抗原表位
- pSer473
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This AKT1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 特异性
- Rabbit Anti-Akt (Phospho-Ser473) detects endogenous human, mouse, and rat Akt1, Akt2 and Akt3, phosphorylated at serine 473, serine 474, and serine 472, respectively.
- 纯化方法
- GenScript Rabbit Anti-Akt (Phospho-Ser473) Polyclonal Antibody is purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide and cross-adsorbed with the corresponding non-phosphopeptide.
- 免疫原
- Synthesized phosphopeptide derived from human Akt around the phosphorylation site of pSer473 (Q-F-SP-Y-S)
- 亚型
- IgG
- Top Product
- Discover our top product AKT1 Primary Antibody
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anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody
AKT1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 122-443) antibodyAKT1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-480) antibodyAKT1 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 1-480) antibodyAKT1 适用: 人 WB, ELISA, IF, IHC (p), IP 宿主: 小鼠 Monoclonal 2E11 unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr450) antibodyAKT1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer124) antibodyAKT1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibodyAKT1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 3E3 unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) antibodyAKT1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibodyAKT1 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibodyAKT1 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, IP, FM, IHC (fp) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-2.0 µg/mL
Other applications: user-optimized - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- PBS, pH 7.4, containing 30 % glycerol, and 0.02 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
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: "Reciprocal regulation of the ubiquitin ligase Itch and the epidermal growth factor receptor signaling." in: Cellular signalling, Vol. 21, Issue 8, pp. 1326-36, (2009) (PubMed).
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: "Reciprocal regulation of the ubiquitin ligase Itch and the epidermal growth factor receptor signaling." in: Cellular signalling, Vol. 21, Issue 8, pp. 1326-36, (2009) (PubMed).
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- 抗原
- AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
- 别名
- Akt (AKT1 产品)
- 别名
- AKT antibody, CWS6 antibody, PKB antibody, PKB-ALPHA antibody, PRKBA antibody, RAC antibody, RAC-ALPHA antibody, Akt antibody, Rac antibody, PKB/Akt antibody, PKBalpha antibody, AKT/PKB antibody, AKT1 antibody, Akt/PKB antibody, Akt1 antibody, CG4006 antibody, D-Akt antibody, DAKT1 antibody, DAKT1/PKB antibody, DAkt antibody, DAkt1 antibody, DPKB antibody, DRAC-PK antibody, DRAC-PK66 antibody, DRAC-PK85 antibody, Dakt antibody, Dakt1 antibody, Dmel\\CG4006 antibody, Dpkb antibody, PKB/AKT antibody, PKB/dAKT antibody, RacPK antibody, akt antibody, akt1 antibody, dAKT antibody, dAKT/dPKB antibody, dAKT1 antibody, dAkt antibody, dAkt/PKB antibody, dAkt1 antibody, dPKB antibody, dakt antibody, dakt1 antibody, l(3)04226 antibody, l(3)89Bq antibody, p-Akt antibody, pAkt antibody, ACT-5 antibody, akt-1 antibody, pkb antibody, v-akt antibody, v-akt1 antibody, xAct antibody, ATAKT1 antibody, F18A8.2 antibody, F18A8_2 antibody, K+ transporter 1 antibody, POTASSIUM TRANSPORTER antibody, AKT serine/threonine kinase 1 antibody, thymoma viral proto-oncogene 1 antibody, CG4006 gene product from transcript CG4006-RE antibody, actin beta antibody, v-akt murine thymoma viral oncogene homolog 1 S homeolog antibody, v-akt murine thymoma viral oncogene homolog 1 antibody, K+ transporter 1 antibody, Serine/threonine-protein kinase akt-1 antibody, AKT1 antibody, Akt1 antibody, ACTB antibody, akt1.S antibody, akt1 antibody, KT1 antibody, akt-1 antibody
- 背景
- Akt, also known as protein kinase B (PKB), a serine/ threonine kinase, is a critical enzyme in several signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. Akt is activated following its phosphorylation at two regulatory residues. Phosphorylation of threonine on the kinase domain, catalyzed by PDK1, is essential for Akt activation. Akt activity is augmented approximately ten fold by phosphorylation at the serine on the hydrophobic motif by PDK2. Phosphorylation of Thr308 and Ser473 activates Akt alpha. Phosphorylation at Thr309 and Ser474 on Akt beta1 and beta2, and on Thr305 on Akt gamma results in their activation. Akt promotes cell survival by inhibiting apoptosis by phosphorylating and inactivating several targets, including bad, forkhead transcription factors, c-Raf and caspase-9. The activation of Akt is negatively regulated by PTEN, a PIP3-specific phosphatase, and SHIP, a SH2-domain containing inositol 5-phosphatase.Rabbit Anti-Akt (Phospho-Ser473) Polyclonal Antibody is developed in rabbit using a synthetic phosphopeptide corresponding to residues surrounding S473 of human, rat, and mouse Akt alpha, also known as Protein Kinase B alpha (PKBalpha), Akt1, and RACalpha. In addition, this phosphopeptide is identical to the sequence surrounding S474 of human, rat, and mouse Akt beta (Akt2) and S472 of human and mouse Akt gamma (Akt3).
- 途径
- PI3K-Akt Signaling, RTK signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect
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