CD4 抗体 (Biotin)
-
- 抗原 See all CD4 抗体
- CD4
-
适用
- 大鼠
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This CD4 antibody is conjugated to Biotin
-
应用范围
- Flow Cytometry (FACS)
- 特异性
- Rat CD4
- 免疫原
- Rat thymocyte membrane . Fusion Partner: P3-NSI-1-Ag4 (NSI/1). . Donor: BALB/c spleen
- 克隆位点
- W3-25
- 亚型
- IgG1
- Top Product
- Discover our top product CD4 Primary Antibody
-
-
- 应用备注
- This clone is reported to work with frozen and paraffin sections (11).
- 限制
- 仅限研究用
-
- 浓度
- 0.1 mg/ml
- 缓冲液
- Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography)
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
-
- 抗原
- CD4
- 别名
- CD4 (CD4 产品)
- 别名
- cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody
- 背景
- Anti-rat CD4 monoclonal antibody recognizes a determinant on the majority of thymocytes (90-95%), a subset of peripheral T cells and peritoneal macrophages. (1,2,3,10.) The antigen recognized by this antibody is a surface glycoprotein of Mr 48,000- 52,000 and is the homologue of the human CD4 and the mouse L3/T4 antigen. ABIN133176labels the rat T helper subset, which mediates the helper activity for B and T cells, graft vs. host (GVH) reactivity and produces IL-2 in the mixed lymphocyte reaction (MLR). (2,4,6,) Addition of ABIN133176to the MLR, inhibited proliferation and blocks the production of IL- 2. (4,5,6,9) T cells which mediate cytotoxicity and suppressor functions are not labelled . (Thus, cells labelled by this antibody are not labelled by MRC OX-8.) ABIN133176is invaluable for separating T cell subsets for functional studies and for labelling cells in tissue sections. It has been used in studing the role of T lymphocytes in graft rejections (7) and in studying the subsets of T cells in the rat which mediate graft vs. host disease.(8) This particular antibody is also one of three antibodies which labels T lymphocyte populations in the rat. These clones include W3/13 ABIN133176, which labels all T cells as well as MRC OX-8 ABIN133176 and W3/25 ABIN133176 which label non-overlapping T cell subpopulations. These monoclonal antibodies used in concert are being employed extensively to investigate cellular aspects of the immune response in rats and prove to be useful as markers for functionally distinct subpopulations of lymphocytes. This clone is reported to work with frozen and paraffin sections (11).
- 途径
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
-