MAP2K3 抗体
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北京 101111
Quick Overview for MAP2K3 抗体 (ABIN126840)
抗原
See all MAP2K3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody specifically recognizes the N-terminus of MKK3.
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产品特性
- Synonyms: MAPKK 3, MEK3, MKK3, MAP kinase kinase 3, Dual specificity mitogen-activated proteinkinase kinase 3
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纯化方法
- Size exclusion chromatography
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组件
- incl. positive Control
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免疫原
- Synthetic peptide conjugated to KLH
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亚型
- IgG1
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应用备注
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Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from untreated A431 cells. ELISA: 0.05 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 1 mL H2O (15 min, RT).
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缓冲液
- 1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C/-80 °C
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储存方法
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch. -
有效期
- 12 months
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- MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))
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别名
- MAP2K3
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背景
- MKK3 activates p38 MAP kinase by phosphorylating a Thr and Tyr residue in the activation loop. MKK3 does not phosphorylate and activate the other major MAP kinases, MAPK1/2 (erk1/2) or SAPK/JNK. Cellular stress and inflammatory cytokines activate MKK3 and lead to phosphorylation of Ser 189 and Thr 193.Synonyms: Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MEK3, MKK3
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基因ID
- 5606
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UniProt
- P46734
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途径
- MAPK Pathway, TLR signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2
抗原
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