ErbB2/Her2 抗体 (pSer1113)
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- 抗原 See all ErbB2/Her2 抗体
- ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))
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抗原表位
- pSer1113
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This ErbB2/Her2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
- 特异性
- This antibody specifically recognizes erbB2 phosphorylated at Serine 1113 at 185 kDa.
- 产品特性
- Synonyms: HER-2, NEU, p185erbB2, NGL, c-erbB-2, MNL19, Receptor tyrosine-protein kinase erbB-2,NEU proto-oncogene, Tyrosine kinase-type cell surface receptor HER2
- 纯化方法
- Subsequent Thiophilic and Size Exclusion Chromatography.
- 组件
- incl. positive Control
- 免疫原
- Phosphopetide conjugated to KLH. Epitope: Phospho-Serine 1113AA Sequence: Q R Y pS E D P
- 克隆位点
- 9-00E-010
- 亚型
- IgG1
- Top Product
- Discover our top product ErbB2/Her2 Primary Antibody
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anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (AA 23-372) antibody
ErbB2/Her2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (C-Term) antibodyErbB2/Her2 适用: 人, 小鼠, 大鼠, 犬, 猴, 马, Cow, Pig, Hamster, 兔, Cat, 小鸡, 驴, 山羊, 豚鼠, 绵羊, Avian WB 宿主: 山羊 Polyclonal unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (AA 22-121) antibodyErbB2/Her2 适用: 人 WB, ELISA, IF, IHC (p), PLA 宿主: 小鼠 Monoclonal 3B1 unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (pTyr1112) antibodyErbB2/Her2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (pTyr1248) antibodyErbB2/Her2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (pTyr877) antibodyErbB2/Her2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (C-Term) antibodyErbB2/Her2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (AA 311-462) antibodyErbB2/Her2 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal ERBB2-3078 unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (AA 311-462) antibodyErbB2/Her2 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal ERBB2-2452 unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (pTyr1248) antibodyErbB2/Her2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA: Use at 0.1 μg/mLImmunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Immunocytochemistry: Use 1-10 μg/mLIncluded Positive Control: Cell lysate from untreated pervanadate-treated A431 cells (SeeProtocols for more details).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Positive Control Provided. Cell lysate from A 431 pervanadate treatedDescription: Cell lysate from pervanadate-treated A431 cells, epidermoid carcinoma(Human)Format: Lyophilized cell lysate from serum starved A431 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 1 mL H2O (15 min, RT).
- 缓冲液
- 1 mLPBS containing 0.09 % Sodium Azide/PEG and Sucrose.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C/-80 °C
- 储存方法
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))
- 别名
- CD340 / ERBB2 / HER2 (ErbB2/Her2 产品)
- 别名
- CD340 antibody, HER-2 antibody, HER-2/neu antibody, HER2 antibody, MLN 19 antibody, NEU antibody, NGL antibody, TKR1 antibody, Erbb-2 antibody, Neu antibody, c-erbB2 antibody, c-neu antibody, mKIAA3023 antibody, wu:fv70f10 antibody, zgc:63601 antibody, erb2 antibody, erb-b2 receptor tyrosine kinase 2 antibody, ERBB2 antibody, Erbb2 antibody, erbb2 antibody
- 背景
- ErbB2 is a member of the EGFR/erbB-receptor tyrosine kinase family. ErbB2 is activated upon ligand dependent heterodimerization with EGFR or erbB4. ErbB2 homodimers are not favored due to the lack of an erbB2 specific extracellular ligand. Heterodimerization with EGFR or erbB4 leads to activation of the intrinsic tyrosine kinase activity of EGFR or erbB4 resulting in phosphorylation of multiple tyrosine residues within the erbB2 intracellular domain: Tyr 1023, Tyr 1112, Tyr 1139, Tyr 1196, Tyr 1222, and Tyr 1248. Transphosphorylation via src family kinases leads to phosphorylation of Tyr 877, via PKC of Thr 686, via CamKinase2 of Ser 1113. Phosphorylation of erbB2 at serine 1113 has been correlated with erbB2 overexpression and a poor prognosis. Thus the phosphorylation status of serine 1113 in erbB2 represents a novel and informative biomarker of cancer cell biology and tumor behavior. (Mol. Cell Biochem. 218: 47-54, 2001).Synonyms: HER-2, MNL19, NEU, NEU proto-oncogene, NGL, Receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, c-erbB-2, p185erbB2
- 基因ID
- 2064
- UniProt
- P04626
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development
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