Glucocorticoid Receptor 抗体 (AA 150-176)
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- 抗原 See all Glucocorticoid Receptor (NR3C1) 抗体
- Glucocorticoid Receptor (NR3C1) (Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1))
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抗原表位
- AA 150-176
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Glucocorticoid Receptor antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- 特异性
- This antibody recognises the glucocorticoid receptor, located in the cytoplasm of cells, and associated with certain heat shock proteins. Clone 8E9 binds to an epitope between amino acids 167-176.
- 纯化方法
- Affinity chromatography on Protein G
- 免疫原
- 26 amino acid peptide corresponding to residues 150-176 of human GCR linked to thyroglobulin (human). Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp-2/0 Ag14 myeloma cell line.
- 克隆位点
- 8-00E-009
- 亚型
- IgG1
- Top Product
- Discover our top product NR3C1 Primary Antibody
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- 应用备注
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ELISA: 1/1000. Western Blot: 1/1000. Successful use in Immunohistochemistry on frozen and paraffin sections was reported bysome researchers. As this detection method has not been verified by antibodies-online, theapplication was deleted from the database. This does not necessarily exclude the use insuch procedure.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.2 containing 0.09 % Sodium Azide and 0.1 % Bovine Serum Albumin
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- Glucocorticoid Receptor (NR3C1) (Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1))
- 别名
- Glucocorticoid Receptor (NR3C1 产品)
- 别名
- GR antibody, gcr antibody, grl antibody, xGR antibody, gccr antibody, gr antibody, zgc:113038 antibody, NR3C1 antibody, GCL antibody, LOC100302444 antibody, Gcr antibody, Grl antibody, Grl-1 antibody, Grl1 antibody, GRL antibody, GCCR antibody, GCR antibody, NO antibody, GR-A antibody, nr3c1 antibody, nuclear receptor subfamily 3 group C member 1 antibody, nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody, nuclear receptor subfamily 3, group C, member 1 antibody, glucocorticoid receptor antibody, NR3C1 antibody, nr3c1 antibody, LOC100302444 antibody, Nr3c1 antibody
- 背景
- Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor.Synonyms: GR, GRL, NR3C1
- 基因ID
- 2908
- NCBI登录号
- NP_000167
- UniProt
- P04150
- 途径
- Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Regulation of Carbohydrate Metabolic Process
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