IL-1 beta 抗体
(4 references)Quick Overview for IL-1 beta 抗体 (ABIN1176987)
抗原
See all IL-1 beta (IL1B) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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品牌
- BD Pharmingen™
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产品特性
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Neutralization Activity: 50% Neutralization (ND50) at 25 - 100 ng/ml
95% Neutralization at 125 - 500 ng/ml -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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过滤
- 0.2 μm filtered
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内毒素水平
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
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免疫原
- Mouse IL-1beta Protein
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亚型
- IgG lambda
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anti-Interleukin 1, beta (IL1B) (AA 101-200) antibody
IL1B 适用: 人, 小鼠, 大鼠, 兔, Cow, Pig WB, ELISA, FACS, IF (p), IHC (p), IF (cc), IHC (fro), DB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1, beta (IL1B) antibodyIL1B 适用: 人 IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 3A6 unconjugated
anti-Interleukin 1, beta (IL1B) (AA 161-269) antibodyIL1B 适用: 人, 小鼠, 大鼠, 兔, Cow WB, ELISA, IF (p), IHC (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1, beta (IL1B) (AA 170-269) antibodyIL1B 适用: 人 WB, ELISA, IP, IF 宿主: 小鼠 Monoclonal 2A8 unconjugated
anti-Interleukin 1, beta (IL1B) (AA 117-269) antibodyIL1B 适用: 人 IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 6F12D5 unconjugated
anti-Interleukin 1, beta (IL1B) (AA 117-269) antibodyIL1B 适用: 人 ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 5C3B7 unconjugated
anti-Interleukin 1, beta (IL1B) (AA 1-266) antibodyIL1B 适用: 绵羊 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1, beta (IL1B) (AA 1-269) antibodyIL1B 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1, beta (IL1B) (AA 148-174) antibodyIL1B 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB21917 unconjugated
anti-Interleukin 1, beta (IL1B) (AA 148-174) antibodyIL1B 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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Neutralization: The NA/LE™ format of this antibody is useful for neutralization of mouse IL-1beta bioactivity. Assay Type: Proliferation at 2 ng/ml mouse IL-1beta (Cat. No. 554577) Indicator cell: TF-1 at 10^5 cells/ml and Preincubation of antibody and ligand = 60 minutes ELISA Capture: The purified B122 antibody (Cat. No. 550605) has been reported to be useful as a capture antibody for a sandwich ELISA for measuring mouse IL-1beta protein levels.
Immunoprecipitation: The B122 antibody has been reported to be useful for the immunoprecipitation of both natural and recombinant forms of mouse IL-1beta protein. Please note that the antibody is not routinely tested for this application at BD Biosciences. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
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储存液
- Azide free
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "Experimental hypersensitivity pneumonitis: in vitro effects of interleukin-2 and interferon-gamma." in: The Journal of laboratory and clinical medicine, Vol. 126, Issue 5, pp. 485-94, (1995) (PubMed).
: "Inhibition of mature IL-1 beta production in murine macrophages and a murine model of inflammation by WIN 67694, an inhibitor of IL-1 beta converting enzyme." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 3, pp. 1331-8, (1995) (PubMed).
: "Generation of monoclonal antibodies to murine IL-1 beta and demonstration of IL-1 in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 5, pp. 1534-40, (1991) (PubMed).
: "IL-1 up-regulates the expression of cytokine receptors on a factor-dependent human hemopoietic cell line, TF-1." in: International immunology, Vol. 3, Issue 6, pp. 571-7, (1991) (PubMed).
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: "Experimental hypersensitivity pneumonitis: in vitro effects of interleukin-2 and interferon-gamma." in: The Journal of laboratory and clinical medicine, Vol. 126, Issue 5, pp. 485-94, (1995) (PubMed).
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- IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
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别名
- IL-1beta
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背景
- The B122 antibody recognizes the precursor and mature secreted forms of the mouse IL-1beta protein. B122 neutralizes the biological activity of mouse IL-1beta, but not the biological activities of mouse IL-1alpha nor human IL-1beta. B122 does not recognize mouse IL-2, IL-4, IL-6, IL-10, TNF or IFN-gamma. This antibody also recognizes rat IL-1beta. The immunogen used to generate the B122 hybridoma was a purified, mature form of the mouse IL-1beta protein.
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途径
- NF-kappaB Signaling, Interferon-gamma Pathway, TLR signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome
抗原
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