ERK2 抗体 (Alexa Fluor 647)
(1 reference)-
- 抗原 See all ERK2 (MAPK1) 抗体
- ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))
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适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This ERK2 antibody is conjugated to Alexa Fluor 647
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应用范围
- Intracellular Staining (ICS)
- 品牌
- BD Phosflow™
- 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human ERK2 Synthetic Peptide
- 克隆位点
- G263-7
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product MAPK1 Primary Antibody
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anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 200-300) antibody
MAPK1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 25-360) antibodyMAPK1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 261-360) antibodyMAPK1 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 1D1 unconjugated
anti-Mitogen-Activated Protein Kinase 1 (MAPK1) antibodyMAPK1 适用: 人, 大鼠, 犬, 猴 WB, IHC, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 6F8 unconjugated
anti-Mitogen-Activated Protein Kinase 1 (MAPK1) antibodyMAPK1 适用: 人, 大鼠, 犬, 猴 WB, IHC, IF, IP, IHC (p) 宿主: 小鼠 Monoclonal 4C2 unconjugated
anti-Mitogen-Activated Protein Kinase 1 (MAPK1) antibodyMAPK1 适用: 人 WB, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal 9E9 unconjugated
anti-Mitogen-Activated Protein Kinase 1 (MAPK1) antibodyMAPK1 适用: 人, 大鼠, 犬, 猴 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 9H1 unconjugated
anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 200-300) antibodyMAPK1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (N-Term) antibodyMAPK1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 310-360) antibodyMAPK1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
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- 样本量
- 20 μL
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
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: "Hyperexpression of mitogen-activated protein kinase in human breast cancer." in: The Journal of clinical investigation, Vol. 99, Issue 7, pp. 1478-83, (1997) (PubMed).
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: "Hyperexpression of mitogen-activated protein kinase in human breast cancer." in: The Journal of clinical investigation, Vol. 99, Issue 7, pp. 1478-83, (1997) (PubMed).
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- 抗原
- ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))
- 别名
- ERK2 (MAPK1 产品)
- 背景
- The members of the Mitogen-Activated Protein Kinase (MAPK) family are components of a key signal transduction cascade that links events at the cell surface to responses in the nucleus. The signaling cascade is found in species as varied as yeast and humans, with many of the proteins being well conserved. In mammals the most widely studied members of the cascade are the Extracellular signal-Regulated Kinases, ERK1 (p44 MAPK) and ERK2 (p42 MAPK). ERK1 and ERK2 share 85% homology and are activated by extracellular signals such as growth factors, hormones, and phorbol esters. Activation occurs through a series of phosphorylations by kinases activating other kinases and eventually leading to phosphorylation of the ERKs. Growth factor stimulation leads to activation of Ras and Raf, leading to phosphorylation of MEK1 (MAPK/ERK kinase) which, in turn, activates the ERKs via dual phosphorylation. Once activated, the ERKs phosphorylate other cytoplasmic signalling molecules (protein kinases and phosphatases), cell-surface receptors, microtubule-associated proteins, and transcription factors in the nucleus. Thus, the active ERK has myriad downstream effectors that implicate it in the control of cell proliferation and differentiation, as well as regulation of the cytoskeleton. Furthermore, studies have shown that elevated ERK activity is associated with some cancers. The G263-7 recognizes ERK2. It does not cross-react with ERK1. Clone G263-7 was originally characterized in human (A431) and mouse (NIH/3T3) cells.A human ERK2 synthetic peptide was used as immunogen.
- 途径
- MAPK Pathway, RTK signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins
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