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IL1RN 抗体

IL1RN 适用: 人, 猴 WB, IHC (p), EIA, IHC (fro), Func 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN116864
发货至: 中国
  • 抗原 See all IL1RN 抗体
    IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))
    适用
    • 78
    • 34
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    • 4
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    • 3
    人, 猴
    宿主
    • 96
    • 33
    • 5
    • 3
    • 1
    • 1
    克隆类型
    • 103
    • 36
    多克隆
    标记
    • 69
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    • 2
    • 2
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    • 2
    • 2
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    This IL1RN antibody is un-conjugated
    应用范围
    • 115
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    • 37
    • 25
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    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)
    纯化方法
    DEAE Chromatography.
    免疫原
    Prepared by repeated immunizations with recombinant human sIL-1Ra produced in E.coli.
    Top Product
    Discover our top product IL1RN Primary Antibody
  • 应用备注
    This IgG fraction antibody of anti-Human IL-1 Receptor Antagonist has been tested for usein Neutralizations, ELISA, Radioimmunoassays, Western blotting, Immunohistochemistryand Flow cytometry. Reactivity in other immunoassays is unknown. Note: This product has been assayed by immunoblot using HRP Goat-anti-Rabbit IgG [H&L]and TMB as a substrate. A working dilution range of 1: 400 to 1: 800 is suggested for thisapplication. This product has been assayed by ELISA against IL-1Receptor Antagonist usingHRP Conjugated Anti-Rabbit IgG [H&L] (Goat) and ABTS as a substrate for 30 minutes atroom temperature. A working dilution range of 1: 500 to 1: 1,000 is suggested for thisproduct. For use in ELISA formats, this antibody is best used as the second antibody incombination with a monoclonal antibody as a capture antibody. This product has beenassayed by Immunohistochemistry. A dilution of 1: 400 or greater is suggested for thisimmunoassay. Either paraffin fixation or cryofixation can be used forImmunohistochemistry. This product has been assayed by radioimmunoassay againstantigen. A dilution of 1: 8,000 is suggested for this immunoassay. Recommended Dilutions: ELISA: 1/1,000-1/5,000. Western Blot: 1/500-1/2,000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    1.0 mg/mL (by UV absorbance at 280 nm)
    缓冲液
    0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
    储存条件
    4 °C/-20 °C
    储存方法
    Store lyophilized product at 2-8 °C. After reconstitution, store at -20 °C.
  • 抗原
    IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))
    别名
    IL1RN / IL1RA (IL1RN 产品)
    别名
    DIRA antibody, ICIL-1RA antibody, IL-1RN antibody, IL-1ra antibody, IL-1ra3 antibody, IL1F3 antibody, IL1RA antibody, IRAP antibody, MVCD4 antibody, IRAP1 antibody, IL1RN antibody, IL-1RA antibody, F630041P17Rik antibody, interleukin 1 receptor antagonist antibody, interleukin 1 receptor antagonist-like antibody, IL1RN antibody, Il1rn antibody, IL1RNL antibody
    背景
    IL1RA is a member of the Interleukin 1 cytokine family. This protein inhibits the activities of Interleukin 1, alpha (IL1A) and Interleukin 1, beta (IL1B), and modulates a variety of Interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.Synonyms: ICIL-1RA, IL-1RN, IL-1ra, IL1 inhibitor, IL1F3, IRAP, Interleukin-1 receptor antagonist protein
    基因ID
    9606
    UniProt
    P18510
    途径
    NF-kappaB Signaling, Hormone Transport, Cancer Immune Checkpoints
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