CIITA 抗体 (C-Term)
-
- 抗原 See all CIITA 抗体
- CIITA (Class II, Major Histocompatibility Complex, Transactivator (CIITA))
-
抗原表位
- AA 726-742, C-Term
-
适用
- 人
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This CIITA antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
- 序列
- CGEIKDNKEL PQYLALTTR
- 特异性
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Cross reactivity against CIITA from other sources may occur but has not been specifically determined.
- 纯化方法
- Multi-step process.
- 免疫原
- Peptide corresponding to a region near the C-terminal end (amino acids 726-742) of the human CIITA gene conjugated to Keyhole Limpet Hemocyanin (KLH).
- Top Product
- Discover our top product CIITA Primary Antibody
-
anti-Class II, Major Histocompatibility Complex, Transactivator (CIITA) (AA 945-1130) antibody
CIITA 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Class II, Major Histocompatibility Complex, Transactivator (CIITA) (Internal Region) antibodyCIITA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Class II, Major Histocompatibility Complex, Transactivator (CIITA) (AA 1) antibodyCIITA 适用: Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Class II, Major Histocompatibility Complex, Transactivator (CIITA) (AA 841-890) antibodyCIITA 适用: 小鼠, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-Class II, Major Histocompatibility Complex, Transactivator (CIITA) (C-Term) antibodyCIITA 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Class II, Major Histocompatibility Complex, Transactivator (CIITA) (AA 650-750) antibodyCIITA 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation,conjugation and most immunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed by immunoblot against CIITAusing Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 1,000 is suggested for this product. Best Results are obtained using 0.03 % (v/v) Triton X-100 IN BUFFERS. Do not supplement buffers with Tween-20 detergent.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
-
- 浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store original vial and reconstituted antibody at -20 °C.
-
- 抗原
- CIITA (Class II, Major Histocompatibility Complex, Transactivator (CIITA))
- 别名
- CIITA / C2TA (CIITA 产品)
- 别名
- CIITA antibody, C2ta antibody, EG669998 antibody, Gm9475 antibody, C2TA antibody, CIITAIV antibody, MHC2TA antibody, NLRA antibody, Mhc2ta antibody, class II, major histocompatibility complex, transactivator antibody, class II major histocompatibility complex transactivator antibody, MHC class II transactivator antibody, class II transactivator antibody, CIITA antibody, LOC710761 antibody, ciita antibody, LOC100443575 antibody, Ciita antibody, LOC100736732 antibody
- 背景
- The transactivator CIITA regulates basal and interferon-induced expression of MHC class II genes. CIITA restores expression of all MHC II gene expression in mutant cells and corrects regulatory defects of those genes. Activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. CIITA may act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter.Synonyms: MHC class II transactivator, MHC2TA
- 基因ID
- 4261
- NCBI登录号
- NP_000237
- UniProt
- P33076
- 途径
- Cancer Immune Checkpoints
-
