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- 抗原 See all CD45 (PTPRC) 抗体
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
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适用
- 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD45 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- This monoclonal antibody recognizes a monomorphic determinant of the rat leukocyte common antigen.
- 纯化方法
- Protein G Chromatography
- 免疫原
- Lymph node glycoproteins and cells. Donor: BALB/c spleen Fusion Partner: NSO/U
- 克隆位点
- OX-30
- 亚型
- IgG2a
- Top Product
- Discover our top product PTPRC Primary Antibody
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anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 1210-1304) antibody
PTPRC 适用: 人, 小鼠, 大鼠, 兔 FACS, WB, IHC (p), ELISA, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibodyPTPRC 适用: 人, 犬 FACS, IHC, WB, StM 宿主: 小鼠 Monoclonal 2B11-PD7-26 unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 193-575) antibodyPTPRC 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 371-556) antibodyPTPRC 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 24-575) antibodyPTPRC 适用: 人 FACS, IHC, WB, IF, ELISA 宿主: 小鼠 Monoclonal 17H9D4 unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (C-Term) antibodyPTPRC 适用: 人, 小鼠, 大鼠, 犬, 小鸡, Cow, Cat, 兔, 绵羊, Pig, 豚鼠, 山羊, 马, 猴, Hamster, 驴, Avian WB 宿主: 山羊 Polyclonal unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibodyPTPRC 适用: 人 FACS, IHC, WB, IF, StM 宿主: 兔 Monoclonal PTPRC-2877R unconjugated Recombinant Antibody
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibodyPTPRC 适用: 人 FACS, WB, IP, IHC (p), ICC 宿主: 小鼠 Monoclonal MEM-28 unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 378-577) antibodyPTPRC 适用: 人, 小鼠, 大鼠 FACS, IHC, WB, ELISA, ICC 宿主: 小鼠 Monoclonal 2H3E2 unconjugated
anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) (AA 431-577) antibodyPTPRC 适用: 人 FACS, IHC, WB, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 7C9F12 unconjugated
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- 应用备注
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Flow cytometry (see protocol). Immunohistochemistry with frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-Rat cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0. 5-1. 0 µg* of this Ab. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (FITC Goat anti-mouse IgG (H+L)) at 1: 700 dilution. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes areprotected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Rat Strain: WistarCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 5 µg/10e6 cellsIsotypic Control: Mouse IgG2aCell Source Percentage of cells stained above control: Thymus: 99. 9%Spleen: 97. 4%Lymph Node: 90. 6%Results - Strain Distribution: Antibody Concentration Used: 0. 5 µg/10e6 cellsStrains Tested: Wistar, Buffalo, Brown Norway, Fischer 344
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS and 0.02 % NaN3
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
- 别名
- CD45 / LCA (PTPRC 产品)
- 别名
- PTPRC antibody, si:dkeyp-47c5.1 antibody, B220 antibody, CD45 antibody, CD45R antibody, GP180 antibody, L-CA antibody, LCA antibody, LY5 antibody, T200 antibody, Cd45 antibody, Ly-5 antibody, Lyt-4 antibody, loc antibody, Lca antibody, RT7 antibody, cd45 antibody, protein tyrosine phosphatase, receptor type C antibody, protein tyrosine phosphatase, receptor type, C antibody, receptor-type tyrosine-protein phosphatase C antibody, PTPRC antibody, ptprc antibody, LOC100074271 antibody, Ptprc antibody
- 背景
- The antigen recognized is a heavily glycosylated membrane glycoprotein of molecular weight 170,000 kDa on thymocytes but molecular weight 170,000-220,000 kDa on other leukocytes. The leukocyte common antigen (L-CA) is a major glycoprotein of haematopoietic cells but is not found on other tissues or erythroid cells. It is present on greater than 95 % of thymocytes, bone marrow cells and thoracic duct lymphocytes. This molecule carries much of the carbohydrate of thymocytes and shows interesting heterogeneity amongst T lymphocytes and B lymphocytesSynonyms: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
- 基因ID
- 24699
- NCBI登录号
- NP_001103357
- UniProt
- P04157
- 途径
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling
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