L-Selectin 抗体 (APC)
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- 抗原 See all L-Selectin (SELL) 抗体
- L-Selectin (SELL) (Selectin L (SELL))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This L-Selectin antibody is conjugated to APC
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
- Monoclonal Anti-Mouse CD62L APC is recommended for use in flow cytometry for identification of L-selectin (CD62L).
- 免疫原
- 38C-13 B cell lymphoma cell lines derived from a C3H/eb mouse. Species
- 克隆位点
- MEL-14
- 亚型
- IgG2a
- Top Product
- Discover our top product SELL Primary Antibody
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anti-Selectin L (SELL) (AA 301-372) antibody
SELL 适用: 小鼠, 大鼠 FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Selectin L (SELL) (AA 1-100) antibodySELL 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Selectin L (SELL) antibody (APC)SELL 适用: 人 FACS 宿主: 小鼠 Monoclonal LT-TD180 APC
anti-Selectin L (SELL) (AA 83-186) antibodySELL 适用: 人 FACS, WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 8C8B7 unconjugated
anti-Selectin L (SELL) (AA 109-346) antibodySELL 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Selectin L (SELL) antibodySELL 适用: 人, Cow FACS, IP, ICC 宿主: 小鼠 Monoclonal IVA94 unconjugated
anti-Selectin L (SELL) antibody (FITC)SELL 适用: 人 FACS 宿主: 小鼠 Monoclonal DREG56 FITC
anti-Selectin L (SELL) antibodySELL 适用: 小鼠 FACS, IP, IHC (fro), ICC, IHC (p) 宿主: 大鼠 Monoclonal Mel-14 unconjugated
anti-Selectin L (SELL) antibody (FITC)SELL 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal Mel-14 FITC
anti-Selectin L (SELL) antibodySELL 适用: 小鼠 FACS, IP, IHC (fro), Func, ICC, IHC (p) 宿主: 大鼠 Monoclonal Mel-14 unconjugated
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- 应用备注
- It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of mouse tissue for flow cytometric analysis using 0,25 µg/10^6 cells
- 说明
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Allophycocyanin (APC). Abs/Em: 651/662 nm).
- 试剂准备
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- Transfer the sample of blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add anti-mouse CD62L APC and mix gently with a vortex mixer. The optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive APC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 mL of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 mL 0.01 mol/L PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis research.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.
- 储存液
- Sodium azide
- 注意事项
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
- 储存条件
- 4 °C
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- 抗原
- L-Selectin (SELL) (Selectin L (SELL))
- 别名
- CD62L (SELL 产品)
- 别名
- CD62L antibody, LAM1 antibody, LECAM1 antibody, LEU8 antibody, LNHR antibody, LSEL antibody, LYAM1 antibody, PLNHR antibody, TQ1 antibody, A.11 antibody, L-selectin antibody, LECAM-1 antibody, SELE antibody, AI528707 antibody, Lnhr antibody, Ly-22 antibody, Ly-m22 antibody, Lyam-1 antibody, Lyam1 antibody, selectin L antibody, selectin, lymphocyte antibody, SELL antibody, Sell antibody
- 背景
- CD62L is a 74-95 kDa glycoprotein also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and na-ve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish na-ve, effector, and memory T cells. CD62L has been reported to interact with CD34, glyCAM-1, and MAdCAM-1.
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