CD44 抗体 (APC)
-
- 抗原 See all CD44 抗体
- CD44
-
适用
- 小鼠
-
宿主
- 大鼠
-
克隆类型
- 单克隆
-
标记
- This CD44 antibody is conjugated to APC
-
应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
- Monoclonal Rat Anti-Mouse CD44 APC is recommended for use in flow cytometry for identification of CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. .
- 免疫原
- Dexamethasone-induced myeloid leukemia M1 cells
- 克隆位点
- IM7
- 亚型
- IgG2b
- Top Product
- Discover our top product CD44 Primary Antibody
-
anti-CD44 (CD44) antibody
CD44 适用: 人, 小鼠 FACS, WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 8E2F3 unconjugated
anti-CD44 (CD44) antibodyKO Validated CD44 适用: 人 WB, IHC (p), IF, IP, IHC (fro), ICC, LCI 宿主: 兔 Polyclonal unconjugated
anti-CD44 (CD44) (AA 21-220) antibodyCD44 适用: 人 FACS, WB, IHC, ELISA, IF 宿主: 小鼠 Monoclonal 6G2D2 unconjugated
anti-CD44 (CD44) antibodyCD44 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD44 (CD44) antibodyCD44 适用: 人 FACS, WB, IHC, ELISA, IHC (p), IP, Inhibition 宿主: 小鼠 Monoclonal MEM-85 unconjugated
anti-CD44 (CD44) antibodyCD44 适用: 人, 犬, Pig FACS, WB, IHC (p), IP 宿主: 小鼠 Monoclonal MEM-263 unconjugated
anti-CD44 (CD44) (AA 346-385) antibodyCD44 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD44 (CD44) (AA 148-247) antibodyCD44 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD44 (CD44) antibodyCD44 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-CD44 (CD44) (AA 36-194) antibodyCD44 适用: 人 FACS, WB, IHC, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 7F4F12 unconjugated
-
- 应用备注
- It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis.
- 说明
-
Allophycocyanin (APC), Abs/Em: 651/662 nm.
- 试剂准备
-
- Transfer the sample of blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add CD44 APC and mix gently with a vortex mixer. The optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive APC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 mL of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 mL 0.01 mol/L PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- The conjugate is provided in liquid form in buffer containing PBS, 1% BSA and 0.09 % Sodium azide, pH 7,4.
- 储存液
- Sodium azide
- 注意事项
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
- 储存条件
- 4 °C
-
- 抗原
- CD44
- 别名
- CD44 (CD44 产品)
- 别名
- CDW44 antibody, CSPG8 antibody, ECMR-III antibody, HCELL antibody, HUTCH-I antibody, IN antibody, LHR antibody, MC56 antibody, MDU2 antibody, MDU3 antibody, MIC4 antibody, Pgp1 antibody, AU023126 antibody, AW121933 antibody, AW146109 antibody, HERMES antibody, Ly-24 antibody, Pgp-1 antibody, CD44A antibody, METAA antibody, RHAMM antibody, XCD44 antibody, cd44 antibody, cdw44 antibody, cspg8 antibody, ecmr-iii antibody, hcell antibody, lhr antibody, mc56 antibody, mdu2 antibody, mdu3 antibody, mic4 antibody, mutch-i antibody, pgp1 antibody, CD44 molecule (Indian blood group) antibody, syndecan 4 antibody, CD44 antigen antibody, CD44 molecule (Indian blood group) S homeolog antibody, CD44 antibody, SDC4 antibody, Cd44 antibody, cd44.S antibody
- 背景
- CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp- 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
- 途径
- Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling
-
