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CD44 抗体

Name: CD44 抗体
Brand: antibodies-online
SKU: ABIN94121
Availability: OutOfStock

CD44 适用: 人, 犬, Pig FACS, WB, IHC (p), IP 宿主: 小鼠 Monoclonal MEM-263 unconjugated

Western Blotting peripheral blood lymphocytes of various species using anti-CD44 (MEM-263).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD44 (MEM-263) purified antibody (concentration in sample 4 μg/mL) GAM APC.

FACS

Flow cytometry analysis of human peripheral blood (lymphocyte gate) using anti-CD44 () PE conjugate.

FACS

Western blotting analysis of CD44 in MOLT-4 cells (lane 1) and HeLa cells (lane 2) using anti-CD44 (MEM-263) purified, non-reducing conditions.

Separation of human CD44 positive lymphocytes (red-filled) from CD44 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD44 (MEM-263) purified antibody (concentration in sample 4 μg/mL) GAM APC.

FACS

5 images

(2 references)

产品编号 ABIN94121

发货至: 中国

Datasheet as PDF

抗原 See all CD44 抗体

CD44
适用
292

152

81

30

25

23

15

11

9

9

8

6

5

4

4

3

2

1

1

1

1

1

1
人, 犬, Pig
宿主
167

124

77

2

1
小鼠
Compatible Secondaries
克隆类型
253

116

2
单克隆
标记
164

42

30

26

18

8

8

6

6

5

5

5

5

5

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4

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3

3

3

3

2

2

2

2

1

1

1

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1

1

1
This CD44 antibody is un-conjugated
应用范围
259

153

92

73

69

65

60

40

34

31

29

13

7

7

6

5

5

3

2

2

2

2

2

2

1

1

1

1

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1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

特异性

The antibody MEM-263 reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.), it is negative on platelets and hepatocytes.

交叉反应（详细）

Human, Porcine, Canine (Dog)

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

免疫原

COS-7 cells (African Green Monkey).

克隆位点

MEM-263

亚型

IgG1

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应用备注

Flow cytometry: Recommended dilution: 4 μg/mL.
Western blotting: Recommended dilution: 2 μg/mL, 60 min on vertical incubator, positive control: Kg-1a human acute leukemia cell lysate, JURKAT human leukemia T-cell lysate, non-reducing conditions, 6 % separating gel.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: uterus, myometrium.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Elashry, Gaertner, Klymiuk, Eldaey, Wenisch, Arnhold: "Characterisation of stemness and multipotency of ovine muscle-derived stem cells from various muscle sources." in: Journal of anatomy, Vol. 239, Issue 2, pp. 336-350, (2021) (PubMed).

Klein, Weisshardt, Kleff, Jastrow, Jakob, Ergün: "Vascular wall-resident CD44+ multipotent stem cells give rise to pericytes and smooth muscle cells and contribute to new vessel maturation." in: PLoS ONE, Vol. 6, Issue 5, pp. e20540, (2011) (PubMed).
抗原

CD44

别名

CD44 (CD44 产品)

别名

CDW44 antibody, CSPG8 antibody, ECMR-III antibody, HCELL antibody, HUTCH-I antibody, IN antibody, LHR antibody, MC56 antibody, MDU2 antibody, MDU3 antibody, MIC4 antibody, Pgp1 antibody, AU023126 antibody, AW121933 antibody, AW146109 antibody, HERMES antibody, Ly-24 antibody, Pgp-1 antibody, CD44A antibody, METAA antibody, RHAMM antibody, XCD44 antibody, cd44 antibody, cdw44 antibody, cspg8 antibody, ecmr-iii antibody, hcell antibody, lhr antibody, mc56 antibody, mdu2 antibody, mdu3 antibody, mic4 antibody, mutch-i antibody, pgp1 antibody, CD44 molecule (Indian blood group) antibody, syndecan 4 antibody, CD44 antigen antibody, CD44 molecule (Indian blood group) S homeolog antibody, CD44 antibody, SDC4 antibody, Cd44 antibody, cd44.S antibody

背景

CD44 Molecule,CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.,PGP-I, HUTCH-I, ECMR-III, Hermes antigen, Hyaluronate receptor, Heparan sulfate proteoglycan, Epican, MC56, MIC4, INLU, LHR

基因ID

960

UniProt

P16070

途径

Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling