CD35 抗体 (FITC)
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- 抗原 See all CD35 (CR1) 抗体
- CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD35 antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
- Monoclonal Mouse Anti-Human CD35 FITC, is recommended for use in flow cytometry for identification of granulocytes, monocytes, B cells, erythrocytes, follicular dendritic cells, subsets of NK and T cells.
- 克隆位点
- UJ11
- 亚型
- IgG1
- Top Product
- Discover our top product CR1 Primary Antibody
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anti-Complement Component Receptor 1 (CD35) (CR1) (AA 1780-2039) antibody
CR1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) antibodyCR1 适用: 人 IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) antibodyCR1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) (AA 420-550) antibodyCR1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) antibodyCR1 适用: 人, Non-Human Primate FACS, WB, IHC (p), IP, IHC (fro), ICC 宿主: 小鼠 Monoclonal E11 unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) antibody (FITC)CR1 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal E11 FITC
anti-Complement Component Receptor 1 (CD35) (CR1) (AA 901-1095) antibodyCR1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) antibodyCR1 适用: 人 IHC, ELISA 宿主: 兔 Monoclonal 9A9 unconjugated Recombinant Antibody
anti-Complement Component Receptor 1 (CD35) (CR1) antibodyCR1 适用: 人 FACS, DNA Mic 宿主: 小鼠 Monoclonal E11 unconjugated
anti-Complement Component Receptor 1 (CD35) (CR1) antibodyCR1 适用: 人, Baboon, Cynomolgus, 猕猴 FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal E11 unconjugated
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- 应用备注
- It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.
- 说明
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Fluorescein isothiocyanate (Molecular Probes).
- 样品收集
- 1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD1a FITC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive FITC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The conjugate is provided in liquid form in buffer containing Antibody Stabilizer Solution, 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.
- 储存液
- Sodium azide
- 注意事项
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. HT-0035-FITC-1 research3. As with any product derived from biological sources, proper handling procedures should be used.
- 储存条件
- 4 °C
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- 抗原
- CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))
- 别名
- CD35 (CR1 产品)
- 别名
- C3BR antibody, C4BR antibody, CD35 antibody, KN antibody, C3DR antibody, CD21 antibody, Cr-1 antibody, Cr-2 antibody, Cr1 antibody, CR1-L antibody, CR2 antibody, CR1 antibody, complement C3b/C4b receptor 1 (Knops blood group) antibody, complement receptor 2 antibody, complement component (3d/Epstein Barr virus) receptor 2 antibody, complement receptor type 1 antibody, CR1 antibody, Cr2 antibody, CR2 antibody, LOC718120 antibody
- 背景
- CD35 is a receptor for C3b and C4b bound to immune complexes. It is a cofactor for specific proteolytic cleavage of C3b and C4b by plasma serine protease, factor I. Accelerates the decay of both the classical and alternative pathway C3 and C5 convertases on cells to which it is bound (intrinsic activity) and nearby cells (extrinsic activity). The cellular function of CD35 is, on leukocytes and tissue sources, CD35 mediates adherence of C4b/C3b coated particles in preparation for phagocytosis. Phagocytosis most often requires the cooperation of CD35 with complement receptor type 3 (CR3, CD11b/CD18) and the Fcg receptors. Primary function of erythrocyte CD35 is mediating the adherence of targets coated with C4b/C3b and their transport to the fixed phagocyte systems of the spleen and liver.
- 途径
- Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity
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