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Tumor Necrosis Factor (TNF) (AA 77-233) (Active) protein (Biotin)

ABIN2181833 产品详细信息, 供应商: Log in to see
蛋白名称
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
产品特性
AA 77-233
29
20
12
11
7
5
5
5
4
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
宿主

122
51
30
15
11
8
7
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
资源
HEK-293 Cells
167
29
15
4
4
3
3
2
2
2
2
1
1
蛋白类型
Recombinant
生物活性
Active
标记
This Tumor Necrosis Factor protein is labelled with Biotin.
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品牌 MABSol®,UltraLys
特异性 The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
产品特性 The product does NOT contain any epitope tags. The protein has a calculated MW of 17.4 kDa. The protein migrates as 18 kDa on a SDS-PAGE gel under reducing (R) condition.
纯度 >95 % as determined by reduced SDS-PAGE.
过滤 0.22 μm filtered
内毒素水平 Less than 1.0 EU per μg by the LAL method.
质量等级 Ultra Sensitive
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA.Immobilized Human TNFR1/CD120a Protein,C-Fc Tag (Catalog # TN1-H5251) at 5 μg/mL (100 μL/well) can bind Human TNF-alpha Protein, Biotinylated (Catalog # TNA-H8211) with a linear range of 0.8-10 ng/mL.

SDS-PAGE: The primary amine of Biotinylated TNFa was labeled by biotin for detection in ELISA, dot blot or western blot using streptavidin or avidin-conjugated probes.
背景 Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.
分子量 17.4 kDa
NCBI登录号 NP_000585
UniProt P01375
研究领域 Inflammation, Cancer, Virology
途径 NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus
说明

A chemically labeled biotinylated protein with ultra sensitivity.
The product is produced using a chemical labeling approach. The primary amines in the side chains of lysine residues and the N-terminus of protein are conjugated with biotins.
Chemical labeling usually results in multiple biotin attachment on a single protein molecule, which could potentially lead to higher detection sensitivity.

限制 仅限研究用
状态 Lyophilized
溶解方式 Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
缓冲液 PBS, pH 7.4
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -20 °C
储存方法 No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
厂商提供的图像
SDS-PAGE (SDS) image for Tumor Necrosis Factor (TNF) (AA 77-233) (Active) protein (Biotin) (ABIN2181833) Biotinylated Human TNF-alpha on SDS-PAGE under reducing (R) condition. The gel was st...
Binding Studies (Bind) image for Tumor Necrosis Factor (TNF) (AA 77-233) (Active) protein (Biotin) (ABIN2181833) Immobilized Humira at 5 µg/mL (100 µl/well) can bind Biotinylated Human TNF-alpha wi...
背景 Dowlati, Herrmann, Swardfager, Liu, Sham, Reim, Lanctôt: "A meta-analysis of cytokines in major depression." in: Biological psychiatry, Vol. 67, Issue 5, pp. 446-57, 2010 (PubMed).