Retinoblastoma 1 抗体 (underphosphorylated)
(6 references)-
- 抗原 See all Retinoblastoma 1 (RB1) 抗体
- Retinoblastoma 1 (RB1)
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抗原表位
- AA 514-610, underphosphorylated
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适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Retinoblastoma 1 antibody is un-conjugated
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应用范围
- Immunoprecipitation (IP)
- 品牌
- BD Pharmingen™
- 交叉反应
- 小鼠
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Recombinant full-length human Rb
- 克隆位点
- G99-549
- 亚型
- IgG1
- Top Product
- Discover our top product RB1 Primary Antibody
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anti-Retinoblastoma 1 (RB1) (C-Term) antibody
RB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma 1 (RB1) (pSer795) antibodyRB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma 1 (RB1) (pSer807) antibodyRB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma 1 (RB1) (pSer780) antibodyRB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma 1 (RB1) (pSer612) antibodyRB1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 3C11 unconjugated
anti-Retinoblastoma 1 (RB1) (pSer807) antibodyRB1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 5H12 unconjugated
anti-Retinoblastoma 1 (RB1) (C-Term) antibodyRB1 适用: 人 WB, IF, IP, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma 1 (RB1) (pSer780) antibodyRB1 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
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- 应用备注
- Applications include immunoprecipitation at 1-2 µg/one million cells. Rb migrates as multiple, closely-spaced bands between approximately 110-116 kDa when sized on denaturing polyacrylamide gels (i.e. by SDS/PAGE). The multiple bands represent different Rb phosphorylation states, the higher molecular weight bands are more highly phosphorylated than the lower molecular weight bands. The level of phosphorylation is cell cycle dependent, and may also be cell type dependent (not all forms are seen in all cell types that express Rb). Gel conditions influence the actual number of bands observed. In cases where optimal band separation is desired, use a 7 to 10% non-gradient long (at least 12 inches) gel. MOLT-4 human leukemia cells (ATCC CRL-1582) are suggested as a positive control.
- 说明
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Related Products: ABIN967389, ABIN967405,
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "The retinoblastoma protein: more than a tumor suppressor." in: Annual review of cell biology, Vol. 10, pp. 1-29, (1995) (PubMed).
: "Expression of the retinoblastoma protein is regulated in normal human tissues." in: The American journal of pathology, Vol. 144, Issue 3, pp. 500-10, (1994) (PubMed).
: "The retinoblastoma protein and BRG1 form a complex and cooperate to induce cell cycle arrest." in: Cell, Vol. 79, Issue 1, pp. 119-30, (1994) (PubMed).
: "Integration of cell cycle control with transcriptional regulation by the retinoblastoma protein." in: Current opinion in cell biology, Vol. 5, Issue 2, pp. 194-200, (1993) (PubMed).
: "Functional analysis of the retinoblastoma gene product and of RB-SV40 T antigen complexes." in: Cancer surveys, Vol. 12, Issue 6, pp. 153-60, (1992) (PubMed).
: "Tumor suppressor genes." in: Science (New York, N.Y.), Vol. 254, Issue 5035, pp. 1138-46, (1991) (PubMed).
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: "The retinoblastoma protein: more than a tumor suppressor." in: Annual review of cell biology, Vol. 10, pp. 1-29, (1995) (PubMed).
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- 抗原
- Retinoblastoma 1 (RB1)
- 别名
- Rb (RB1 产品)
- 别名
- OSRC antibody, RB antibody, p105-Rb antibody, pRb antibody, pp110 antibody, Rb antibody, Rb-1 antibody, P105-RB antibody, PP110 antibody, RB11 antibody, zgc:154147 antibody, RB transcriptional corepressor 1 antibody, RB transcriptional corepressor like 1 antibody, retinoblastoma 1 antibody, RB1 antibody, RBL1 antibody, Rb1 antibody, rb1 antibody
- 背景
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The retinoblastoma gene encodes a nuclear phosphoprotein (Rb or p110Rb) which is expressed in most normal cells of vertebrates and acts as a tumor suppressor gene product. An underphosphorylated form of Rb is mainly found in resting or fully differentiated cells, whereas the hyperphosphorylated form is present in proliferating cells. Only the underphosphorylated form of Rb binds specifically to viral oncogenes such as SV40 large T, adenoviral EIA and HPV-E7. This interaction may partially contribute to the transforming activity of these viral oncoproteins. Rb also interacts with several cyclins including A, D, and E as well as the transcriptional activator E2F. The importance of these interactions for the biological function of Rb is still being elucidated. G99-549 recognizes only the fast migrating, underphosphorylated form of Rb protein. G99-549 cross-reacts with mouse underphosphorylated Rb. The epitope has been mapped to amino acids 514-610 (box A) of human Rb. Recombinant full-length human Rb was produced in the baculovirus expression vector system (BEVS), purified and used as immunogen.
Synonyms: RB (a.a. 514-610) - 分子量
- 105 kDa
- 途径
- Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy
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