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Retinoblastoma 1 抗体 (pSer780)

Name: Retinoblastoma 1 抗体 (pSer780)
SKU: ABIN6256422
Availability: OutOfStock

RB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

Western blot analysis of Retinoblastoma phosphorylation expression in serum treated K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. (Retinoblastoma 1 抗体 (pSer780))

IF/ICC

ABIN6267315 at 1/200 staining human kidney tissue sections by IHC-P. (Retinoblastoma 1 抗体 (pSer780))

IHC

Western blot analysis of Phospho-Retinoblastoma (Ser780) expression in various lysates (Retinoblastoma 1 抗体 (pSer780))

4 images

产品编号 ABIN6256422

发货至: 中国

Datasheet as PDF

抗原 See all Retinoblastoma 1 (RB1) 抗体

Retinoblastoma 1 (RB1)
抗原表位
31

31

29

24

24

22

20

15

11

10

10

7

6

6

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer780
适用
290

112

94

5

4

4

3

3

3

2

1

1

1
人, 小鼠, 大鼠
宿主
251

42
兔
Compatible Secondaries
克隆类型
237

56
多克隆
标记
146

19

17

16

11

11

6

6

6

6

6

6

6

6

6

6

6

1

1

1

1

1

1

1
This Retinoblastoma 1 antibody is un-conjugated
应用范围
222

131

113

80

79

42

39

34

28

19

19

8

8

6

6

2

2
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

特异性

Phospho-Retinoblastoma (Ser780) Antibody detects endogenous levels of Retinoblastoma only when phosphorylated at Serine 780.

预测反应

Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

纯化方法

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

免疫原

A synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Ser780.

亚型

IgG

Top Product

Discover our top product RB1 Primary Antibody
anti-Retinoblastoma 1 (RB1) (pSer780) antibody
RB1 适用: 人, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (pSer795) antibody
RB1 适用: 人 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (C-Term) antibody
RB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (pSer795) antibody
RB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (pSer807) antibody
RB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (pSer612) antibody
RB1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 3C11 unconjugated

anti-Retinoblastoma 1 (RB1) (pSer807) antibody
RB1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 5H12 unconjugated

anti-Retinoblastoma 1 (RB1) (C-Term) antibody
RB1 适用: 人 WB, IF, IP, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma 1 (RB1) (pSer780) antibody
RB1 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated

anti-Retinoblastoma 1 (RB1) (pSer807), (pSer811) antibody
RB1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C. Stable for 12 months from date of receipt.

有效期

12 months
抗原

Retinoblastoma 1 (RB1)

别名

RB1 (RB1 产品)

别名

OSRC antibody, RB antibody, p105-Rb antibody, pRb antibody, pp110 antibody, Rb antibody, Rb-1 antibody, P105-RB antibody, PP110 antibody, RB11 antibody, zgc:154147 antibody, RB transcriptional corepressor 1 antibody, RB transcriptional corepressor like 1 antibody, retinoblastoma 1 antibody, RB1 antibody, RBL1 antibody, Rb1 antibody, rb1 antibody

背景

Description: Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Gene: RB1

分子量

106kDa

基因ID

5925

UniProt

P06400

途径

Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy