ELISA: Use at a concentration range of 1-2 µg/mL. Western blot: A suitable range of concentrations of this antibody for WB detection is 10 µg/mL. IHC: A suitable range of concentrations of this antibody for IHC is 10 µg/mL. Immunohistochemical analysis of BRAF expression in paraffin embedded formalin fixed Rat brain tissue. ABIN1685167 was used as primary antibody, dilution 1:200.
限制
仅限研究用
状态
Lyophilized
溶解方式
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
缓冲液
PBS solution at a concentration of 1 mg/mL.
注意事项
Avoid repeated freeze/thaw cycles.
储存条件
-20 °C
储存方法
Recombinant human CD117, as supplied, can be stored in working aliquots at - 8 °C for one month, or at -20 °C to -70 °C for twelve months.
The c-Kit receptor consists of an extracellular portion with five immunoglobulin-like regions and an intracellular tyrosine kinase domain. The first three immunoglobulin-like domains form a binding site for its ligand, stem cell factor (SCF), the fourth is involved in c-Kit receptor dimerization, and the function of the fifth is, as yet, unknown. Binding of SCF induces homodimerization of the c-Kit receptor, followed by an increase in receptor autophosphorylation activity and recruitment of the c-Kit signaling complex.