This antibody recognises OX40 (CD134), a cell surface antigen expressed only by activated T lymphocytes. This product is routinely tested on concanavalin A activated splenocytes.
纯化方法
Affinity chromatography on Protein G
免疫原
CHO cells transfected with murine OX40. Spleen cells from immunised AO rats were fused with cells of the mouse NS1 myeloma cell line.
Flow Cytometry: Use 10 μL of neat antibody to label 1x10^6 cells. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with distilled water.
缓冲液
PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
This product is photosensitive and should be protected from light
储存条件
4 °C
储存方法
Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!
抗原
TNFRSF4
(Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))
The OX40 receptor is also known as CD134, ACT35, and tumour necrosis factor receptor superfamily member 4. This receptor is expressed on activated CD4+ and CD8+ T cells and B cells. The OX40 receptor binds to the OX40 ligand to provide a co-stimulatory signal that is independent of CD28. Blockade of OX40-OX40 ligand interactions has been shown to ameliorate experimental EAE and inflammatory bowel disease, imply that these interactions are important in the pathogenesis of some autoimmune diseases.Synonyms: ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, TNFRSF4, TXGP1L, Tumor necrosis factor receptor superfamily member 4