anti-人 WNT9A 抗体 for Immunohistochemistry

Recommended WNT9A Antibody (supplied by: Log in to see )

抗原
Wingless-Type MMTV Integration Site Family, Member 9A (WNT9A) 抗体
  • zgc:153617
  • wnt-9a
  • Wnt14
  • WNT14
  • WNT-14
  • Wnt family member 9A
  • wingless-type MMTV integration site family, member 9A
  • wingless-type MMTV integration site family member 9A S homeolog
  • WNT9A
  • wnt9a
  • wnt9a.S
  • Wnt9a
抗原表位
N-Term
12
10
9
8
3
3
2
2
1
1
1
1
适用
人, 猴
71
28
23
宿主

61
9
克隆类型
多克隆
标记
This WNT9A antibody is un-conjugated
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
41
25
13
11
9
7
2
1
1
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产品编号 ABIN1049488
$ 484.00
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.402891 ABIN1049487 IHC IHC (p) Rabbit Internal Region Log in to see Polyclonal 0
8.402891 ABIN5966063 IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN3046621 IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2390319 IHC WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN6095400 ELISA IHC Rabbit IgG AA 206-365 Log in to see Polyclonal 0
1 ABIN2390310 IHC Rabbit N-Term Log in to see Polyclonal 0
1 ABIN2390309 IHC Rabbit Log in to see Polyclonal 0
1 ABIN6150259 IHC WB Rabbit Log in to see Polyclonal 0
1 ABIN1856239 IHC ELISA WB Rabbit Log in to see Polyclonal 0

General

抗原 Wingless-Type MMTV Integration Site Family, Member 9A (WNT9A) 抗体
抗原表位 N-Term
(12), (10), (9), (8), (3), (3), (2), (2), (1), (1), (1), (1)
适用 人, 猴
(71), (28), (23)
宿主
(61), (9)
克隆类型
多克隆
标记 This WNT9A antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
应用范围 Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(41), (25), (13), (11), (9), (7), (2), (1), (1)
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WNT9A抗体详情

WNT9A目标详情 使用细节 贮存及处理 图像
品牌 IHC-plus™
特异性 Human WNT9A. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
预测反应 Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda (100%) Mouse, Rat, Bovine, Bat, Rabbit (94%).
纯化方法 Immunoaffinity purified
免疫原 Synthetic 16 amino acid peptide from N-Terminus of human WNT9A. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda (100%), Mouse, Rat, Bovine, Bat, Rabbit (94%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

WNT9A目标详情

WNT9A抗体详情 使用细节 贮存及处理 图像 回页首
抗原
别名 WNT14 / WNT9A (WNT9A Antibody 摘要)
背景 Name/Gene ID: WNT9A
Family: WNT

Synonyms: WNT9A, Protein Wnt-14, Protein Wnt-9a, WNT14
基因ID 7483
途径 WNT signaling

使用细节

WNT9A抗体详情 WNT9A目标详情 贮存及处理 图像 回页首
应用备注 Approved: IHC, IHC-P (10 μg/mL)
说明

Target Species of Antibody: Human

实验流程 The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
限制 仅限研究用

贮存及处理

WNT9A抗体详情 WNT9A目标详情 使用细节 图像 回页首
状态 Liquid
浓度 Lot specific
缓冲液 PBS, less than 0.1 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C,-20 °C
储存方法 Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
有效期 12 months

图像

WNT9A抗体详情 WNT9A目标详情 使用细节 贮存及处理 回页首
厂商提供的图像
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family, Member 9A (WNT9A) (N-Term) antibody (ABIN1049488) Anti-WNT9A antibody ABIN1049488 IHC staining of human brain, cortex. Immunohistochem...
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family, Member 9A (WNT9A) (N-Term) antibody (ABIN1049488) Anti-WNT9A antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixe...