We are preparing the requested document. Please wait, this may take a while...!
|抗原||Calcium/calmodulin-Dependent Protein Kinase (CaM Kinase) II beta (CAMK2B) 抗体|
|适用||小鸡, 牛, 犬, 马, 人, 猴, 小鼠, 猪, 兔, 大鼠, 非洲爪蟾, 斑马鱼 其他选择|
|标记||This CAMK2B antibody is un-conjugated 其他选择|
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|Supplier||Log in to see|
CAMK2B抗体详情CAMK2B目标详情 使用细节 贮存及处理 图像
|特异性||Detects proteins of 50-60 kD, corresponding to apparent molecular mass of CaMKII isoforms on SDS-PAGE immunoblots. Recombinant rat calmodulin-dependent protein kinase II alpha subunits expressed in sf9 insect cells are also detected. As the sequences of the rat alpha, delta, and gamma isoforms are conserved over this amino terminus region, this antibody is expected to recognize the delta, gamma and alpha isoforms. This antibody is not expected to cross-react with the beta isoform. Proteins of unknown identity, ~40, 45 and 90 kD, may be detected on immunoblot analysis with some lysates.|
|预测反应||Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Chicken, Xenopus, Pufferfish, Zebrafish (100%) Orangutan (93%).|
A 14 residue synthetic peptide CTRFTDEYQLFEEL based on the rat multifunctional calmodulin-dependent kinase II delta isoform (residues 7-20) and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Chicken, Xenopus, Pufferfish, Zebrafish (100%), Orangutan (93%).
Type of Immunogen: Synthetic peptide - KLH conjugated
|Plasmids, Primers & others|
CAMK2B目标详情CAMK2B抗体详情 使用细节 贮存及处理 图像 回页首
|别名||CAMK2 / CAMKII (CAMK2B Antibody 摘要)|
Name/Gene ID: CAMK2
|途径||WNT signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity|
使用细节CAMK2B抗体详情 CAMK2B目标详情 贮存及处理 图像 回页首
Approved: IHC, IHC-P (10 μg/mL), WB (1:1000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.
Target Species of Antibody: Rat
贮存及处理CAMK2B抗体详情 CAMK2B目标详情 使用细节 图像 回页首
|缓冲液||BBS, pH 8.2 (25 mM sodium borate, 100 mM boric acid, 75 mM sodium chloride, 5 mM EDTA)|
|储存方法||Store at -20°C.|
图像CAMK2B抗体详情 CAMK2B目标详情 使用细节 贮存及处理 回页首