ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1 % cross-reactivity against other mouse heavy or light chain isotypes.
产品特性
Mouse IgG2a secondary antibody is available in a variety of formats. HRP Conjugated Secondary Antibodies are ideal for western blot and are available in a variety of formats and conjugate types. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment compositions.
纯化方法
Preadsorption: Solid phase absorption
免疫原
Mouse IgG2a heavy chain Immunogen Type: Native Protein
Anti-Mouse IgG2a Peroxidase Secondary antibodies are suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG2a antibody based assays.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
浓度
1.0 mg/mL
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
储存液
Gentamicin sulfate
注意事项
Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. Centrifuge product if not completely clear after standing at room temperature.
储存条件
4 °C/-20 °C
储存方法
Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.