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山羊 anti-小鼠 IgG2a (Heavy Chain) Antibody (FITC) - Preadsorbed

Name: 山羊 anti-小鼠 IgG2a (Heavy Chain) Antibody (FITC) - Preadsorbed
Brand: antibodies-online
SKU: ABIN1118478
Availability: OutOfStock

FACS, IHC, WB, FLISA, FM Polyclonal IgG FITC

Dot Blot of Goat anti-Mouse IgG2a Antibody Fluorescein Conjugated Pre-absorbed. (山羊 anti-小鼠 IgG2a (Heavy Chain) Antibody (FITC) - Preadsorbed)

1 image

(3 references)

产品编号 ABIN1118478

发货至: 中国

Datasheet as PDF

抗原 See all IgG2a products

IgG2a
抗原表位
42

15

7

2

2

1

1
Heavy Chain
适用
89

18

1
小鼠
宿主
56

23

13

11

5
山羊
克隆类型
71

24
多克隆
标记
20

11

11

10

8

5

5

4

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
FITC
应用范围
50

42

34

29

23

18

10

8

7

5

4

4

3

3

2

2

2

1

1
Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

特异性

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1 % cross-reactivity against other mouse heavy or light chain isotypes.

交叉反应

小鼠

产品特性

Mouse IgG2a secondary antibody is available in a variety of formats. FITC Conjugated Secondary Antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

纯化方法

Preadsorption: Solid phase absorption

免疫原

Immunogen: Mouse IgG2a heavy chain
Immunogen Type: Native Protein

亚型

IgG

Labeling Ratio

5.2
Goat anti-Mouse IgG2a (Heavy Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP

Rabbit anti-Mouse IgG2a (Heavy Chain) antibody - Preadsorbed
ELISA, IHC, WB Polyclonal IgG unconjugated

Rat anti-Mouse IgG2a antibody
ELISA, FACS Monoclonal SB84a IgG1 unconjugated

Rat anti-Mouse IgG2a antibody (PE)
ELISA, FACS Monoclonal SB84a IgG1 PE

Rat anti-Mouse IgG2a antibody (HRP)
ELISA, IHC Monoclonal SB84a IgG1 HRP

Rabbit anti-Mouse IgG2a (Heavy Chain) antibody (FITC) - Preadsorbed
FACS, FLISA, FM Polyclonal IgG FITC

Goat anti-Mouse IgG2a (Heavy Chain) antibody (TRITC)
ELISA, FACS, IHC Polyclonal IgG TRITC

Rabbit anti-Mouse IgG2a (Heavy Chain) antibody (Alkaline Phosphatase (AP)) - Preadsorbed
ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)

Goat anti-Mouse IgG2a antibody (PE) - Preadsorbed
ELISA, FACS Polyclonal IgG PE

Goat anti-Mouse IgG2a antibody (APC) - Preadsorbed
ELISA, FACS Polyclonal IgG APC

应用备注

Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Mouse IgG2a secondary antibody is available in a variety of formats. FITC Conjugated Secondary Antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:200 - 1:500
Western Blot Dilution: 1:2,000 - 1:10,000
IF Microscopy Dilution: 1:1,000 - 1:5,000

说明

Excitation/Emission wavelength: 494 nm/514 nm

限制

仅限研究用
溶解方式

Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)

浓度

1.7 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Product is photosensitive and should be protected from light.
Centrifuge product if not completely clear after standing at room temperature.

储存条件

RT,4 °C,-20 °C

储存方法

Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

有效期

12 months
Musante, Li, Kanyo, Lam, Colangelo, Cheng, Brody, Greengard, Le Novère, Nairn: "Reciprocal regulation of ARPP-16 by PKA and MAST3 kinases provides a cAMP-regulated switch in protein phosphatase 2A inhibition." in: eLife, Vol. 6, (2018) (PubMed).

Carlyle, Kitchen, Kanyo, Voss, Pletikos, Sousa, Lam, Gerstein, Sestan, Nairn: "A multiregional proteomic survey of the postnatal human brain." in: Nature neuroscience, Vol. 20, Issue 12, pp. 1787-1795, (2017) (PubMed).

Andrade, Musante, Horiuchi, Matsuzaki, Brody, Wu, Greengard, Taylor, Nairn: "ARPP-16 Is a Striatal-Enriched Inhibitor of Protein Phosphatase 2A Regulated by Microtubule-Associated Serine/Threonine Kinase 3 (Mast 3 Kinase)." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 37, Issue 10, pp. 2709-2722, (2017) (PubMed).
抗原

IgG2a

Abstract

IgG2a 产品

物质类

Antibody

背景

Synonyms: goat anti-MOUSE IgG2a Antibody fluorescein Conjugation, goat anti-Mouse IgG2a (gamma 2a) FITC Conjugated Antibody
Background: Anti-Mouse IgG2a Fluorescein Antibody generated in goat detects reactivity to Mouse IgG2a (Gamma 2a chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.