山羊 anti-小鼠 IgG2a (Heavy Chain) Antibody (TRITC) - Preadsorbed
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- 抗原 See all IgG2a products
- IgG2a
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抗原表位
- Heavy Chain
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- TRITC
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应用范围
- Immunohistochemistry (IHC), Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- 特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1 % cross-reactivity against other mouse heavy or light chain isotypes.
- 交叉反应
- 小鼠
- 产品特性
- Mouse IgG2a secondary antibody is available in a variety of formats. Rhodamine secondaaries are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Mouse IgG2a heavy chain
Immunogen Type: Native Protein
- 亚型
- IgG
- Labeling Ratio
- 3.1
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Goat anti-Mouse IgG2a (Heavy Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP
Rabbit anti-Mouse IgG2a (Heavy Chain) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated
Goat anti-Mouse IgG2a (Heavy Chain) antibody (FITC) - PreadsorbedFACS, IHC, WB, FLISA, FM Polyclonal IgG FITC
Rat anti-Mouse IgG2a antibody (HRP)ELISA, IHC Monoclonal SB84a IgG1 HRP
Rabbit anti-Mouse IgG2a (Heavy Chain) antibody (FITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG FITC
Goat anti-Mouse IgG2a (Heavy Chain) antibody (TRITC)ELISA, FACS, IHC Polyclonal IgG TRITC
Rabbit anti-Mouse IgG2a (Heavy Chain) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Mouse IgG2a antibody (FITC)ELISA, FLISA Polyclonal IgG FITC
Goat anti-Mouse IgG2a antibody (PE-Cy7) - PreadsorbedELISA, FACS, FLISA Polyclonal IgG PE-Cy7
Goat anti-Mouse IgG2a antibody (Alkaline Phosphatase (AP))ELISA, FLISA Polyclonal IgG Alkaline Phosphatase (AP)
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- 应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Mouse IgG2a secondary antibody is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG2a antibody based assays. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
IF Microscopy Dilution: 1:1,000 - 1:5,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Avoid cycles of freezing and thawing.
Product is photosensitive and should be protected from light.
Centrifuge product if not completely clear after standing at room temperature. - 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
- 有效期
- 12 months
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- 抗原
- IgG2a
- Abstract
- IgG2a 产品
- 物质类
- Antibody
- 背景
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Synonyms: Goat anti-mouse IgG2a antibody rhodamine conjugation, goat anti-mouse IgG2a (gamma 2a) TRITC conjugated antibody
Background: Anti-Mouse IgG2a Antibody generated in goat detects reactivity to Mouse IgG2a (Gamma 2a chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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