anti-小鼠 PTF1A 抗体 for Intracellular Staining

Recommended PTF1A Antibody (supplied by: Log in to see )

抗原
Pancreas Specific Transcription Factor, 1a (PTF1A) 抗体
  • PTF1A
  • PTF1
  • si:zc142h2.2
  • zgc:112216
  • Ptf1a/p48
  • XPtf1a
  • PTF1-p48
  • bHLHa29
  • PTF1p48
  • pancreas specific transcription factor, 1a
  • PTF1A
  • ptf1a
  • Ptf1a
适用
人, 小鼠, 大鼠
122
32
23
19
7
6
5
3
3
1
1
1
宿主
小鼠
93
34
10
克隆类型
单克隆
标记
This PTF1A antibody is un-conjugated
13
11
10
8
8
8
1
1
1
1
1
1
1
1
1
1
1
应用范围
BioImaging (BI), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Intracellular Staining (ICS), Western Blotting (WB)
107
89
28
13
8
6
4
3
2
1
1
选项
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产品编号 ABIN2689886
产品在该地区无法提供。

General

抗原 Pancreas Specific Transcription Factor, 1a (PTF1A) 抗体
适用 人, 小鼠, 大鼠
(122), (32), (23), (19), (7), (6), (5), (3), (3), (1), (1), (1)
宿主 小鼠
(93), (34), (10)
克隆类型 (克隆位点)
单克隆   ( )
标记 This PTF1A antibody is un-conjugated
(13), (11), (10), (8), (8), (8), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
应用范围 BioImaging (BI), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF), Intracellular Staining (ICS), Western Blotting (WB)
(107), (89), (28), (13), (8), (6), (4), (3), (2), (1), (1)
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PTF1A抗体详情

PTF1A目标详情 使用细节 贮存及处理 图像
品牌 BD Pharmingen™
产品特性 The S25-763 monoclonal antibody specifically binds PTF1A (Pancreas specific transcription factor 1a). PTF1A is a transcription factor that contains a basic helix-loop-helix motif domain and, in combination with p75 and p64, forms the pancreas transcription factor complex. PTF1A plays a roles in pancreatic and cerebellar development. In early pancreatic development, pancreatic bud cells can either continue to pancreatic organogenesis or regress to a duodenal fate, and PTF1A plays a role in this differentiation. In late pancreatic development, the expression of PTF1A is restricted to acinar cells where it is involved in the maintenance of exocrine-specific gene expression. PTF1A expression is often evaluated to monitor pancreatic lineage differentiation from pluripotent stem cells. In addition, PTF1A is expressed during retinal development, and mutations in PTF1A cause cerebellar deficiencies. This mouse anti-human PTF1A monoclonal antibody, clone S25-763, has been demonstrated to cross-react with rat and mouse pancreatic acinar cells. Analysis of PTF1A expression in rat pancreatic acinar cells by western blot and flow cytometry. Left panel: Lysates of the AR42J cell line (ATCC CRL-1492) were electrophoresed (SDS-PAGE) in a 4-20 % Tris-Glycine polyacrylamide gel, transferred to PVDF membranes and then probed with 2 (lane 1), 1 (lane 2), 0.5 (lane 3), 0.25 (lane 4) mg/mL of Purified Mouse anti-PTF1A. Specific staining was detected with HRP Goat Anti-Mouse Ig (Cat. No. 554002). Right panel: AR42J cells were fixed in BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050) and stained with matching concentrations of either Purified Mouse IgG1, κ Isotype Control (Cat. No. 554121, dashed line) or Purified Mouse anti-PTF1A monoclonal antibody (solid line). The second-step reagent was PE Goat Anti-Mouse Ig (550589). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact AR42J cells. Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System. Immunohistochemical analysis of PTF1A expression by acinar cells within the human pancreas. Following antigen retrieval with BD Retrievagen A Buffer (Cat. No. 550524), the formalin-fixed paraffin-embedded sections were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No. 550878, left panel) or Purified Mouse anti-PTF1A antibody (right panel). A three-step staining procedure that employs Biotin Goat Anti-Mouse Ig (Cat. No. 550337), Streptavidin- HRP (Cat. No. 550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. The acinar cell nuclei and cytoplasm stained positively for the expression of PTF1A. Original magnification: 40x.

BD Pharmingen™ Purified Mouse Anti-PTF1A - Purified - Clone S25-763 - Isotype Mouse IgG1, κ - Reactivity Rat, Hu, Ms - 0.1 mg
纯化方法 The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
免疫原 Human PTF1A Recombinant Protein
克隆位点 S25
亚型 IgG1, kappa
Plasmids, Primers & others

PTF1A目标详情

PTF1A抗体详情 使用细节 贮存及处理 图像 回页首
抗原
别名 PTF1A (PTF1A Antibody 摘要)
背景 Synonyms: PTF1α, Ptf1a, Ptf1α, bHLHa29, PTF1-p48
分子量 48 kDa
途径 Retinoic Acid Receptor Signaling Pathway

使用细节

PTF1A抗体详情 PTF1A目标详情 贮存及处理 图像 回页首
应用备注 Optimal working dilution should be determined by the investigator.
限制 仅限研究用

贮存及处理

PTF1A抗体详情 PTF1A目标详情 使用细节 图像 回页首
浓度 0.5 mg/mL
缓冲液 Aqueous buffered solution containing ≤0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
储存方法 Store undiluted at 4°C.