TNF alpha 蛋白
(1 reference)Quick Overview for TNF alpha 蛋白 (ABIN935843)
抗原
See all TNF alpha 蛋白蛋白类型
生物活性
宿主
资源
纯度
-
-
序列
- VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL
-
产品特性
-
Purified recombinant Human TNF alpha protein
Expression System: E.coli
Bioactivity: The ED50 as determined by the cytolysis of Mouse L929 cells in the presence of Actinomycin D is ? 0.05 ng/mL, corresponding to a specific activity of ? 2 x 10^7 units/mg. -
内毒素水平
- < 0.1 ng per μg (1 EU/μg).
-
-
想要此Protein的其他选项吗？
!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 HEK-293 CellsConjugate His tagOrigin Human价格从 136,947.45 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
-
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His-Avi Tag,Biotin)
TNF alpha 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His-Avi Tag)TNF alpha 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His tag)TNF alpha 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) protein (His tag)custom-made TNF alpha 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Tumor Necrosis Factor alpha (TNF alpha) (AA 1-235) protein (His tag)custom-made TNF alpha 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) proteinHPLC verified TNF alpha 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (Biotin)TNF alpha 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (His tag,AVI tag,Biotin)TNF alpha 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (Active) proteinTNF alpha 宿主: 兔 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by reducing SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (Active) proteinTNF alpha 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
-
-
应用备注
- Each Investigator should determine their own optimal working dilution for specific applications.
-
说明
-
Biological activity: The ED50 as determined by the cytolysis of Mouse L929 cells in the presence of Actinomycin D is approx. 0.05 ng/mL, corresponding to a specific activity of approx. 2 x 10^7 units/mg.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Reconstitute in water to a concentration of 0.1 - 1.0 mg/mL. Allow to sit at room temperature for 2 hours before use.
-
缓冲液
- Lyophilized from 3 mM Tris, pH 8.0.
-
注意事项
- Avoid repeated freeze/thaw cycles.
-
储存条件
- 4 °C/-20 °C
-
储存方法
- Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
-
-
-
: "Impaired primary immune response in type-1 diabetes. Functional impairment at the level of APCs and T-cells." in: Cellular immunology, Vol. 221, Issue 1, pp. 15-26, (2003) (PubMed).
-
: "Impaired primary immune response in type-1 diabetes. Functional impairment at the level of APCs and T-cells." in: Cellular immunology, Vol. 221, Issue 1, pp. 15-26, (2003) (PubMed).
-
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
-
别名
- TNF alpha
-
背景
-
TNF-alpha is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes.
Alternative Names: TNFSF2 protein, Necrosin protein, TNF-a protein, Cachectin protein, Cytotoxin protein, TNFa protein, DIF protein, Tumor Necrosis Factor protein, TNF-alpha protein, Differentiation-inducing factor protein -
分子量
- 17.4 kDa
-
途径
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
抗原
-
