PARP1 蛋白
-
- 抗原 See all PARP1 蛋白
- PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))
- 蛋白类型
- Recombinant
-
宿主
- 人
-
资源
- Insect cells (Sf9)
- 产品特性
- ~1,018 U/mg protein. One unit synthesizes 1 nmole of poly(ADP-ribose) per min. at 25°C, pH 7.5.
- 纯化方法
- Affinity purified
- 纯度
- > 99 % as determined by SDS-PAGE.
- Top Product
- Discover our top product PARP1 蛋白
-
Poly (ADP-Ribose) Polymerase 1 (PARP1) (Active) protein
PARP1 宿主: 人 宿主: Baculovirus Recombinant > 95 % WB, ELISA, PAR Active
Poly (ADP-Ribose) Polymerase 1 (PARP1) (Internal Region) proteinPARP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Ct
Poly (ADP-Ribose) Polymerase 1 (PARP1) proteinPARP1 宿主: 人 宿主: Baculovirus Recombinant > 95 % WB, ELISA
Poly (ADP-Ribose) Polymerase 1 (PARP1) (N-Term) proteinPARP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Ct
Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 787-1014) protein (His-IF2DI Tag)PARP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 1-100) protein (GST tag)PARP1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 662-1014) proteinPARP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS
Poly (ADP-Ribose) Polymerase 1 (PARP1) (Active) protein (His tag)PARP1 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 85 % as determined by SDS-PAGE Active
Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 2-1014) protein (His tag)Crystallography grade PARP1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 2-1013) protein (His tag)Crystallography grade PARP1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
-
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- 20 µg at 1 mg/mL affinity-purified liquid human recombinant PARP-1 in 100 mM Tris-HCl (pH 7.5) containing 14 mM -mercaptoethanol, 0.5 mM EDTA, 0.5 mM PMSF and 10% glycerol.
- 储存条件
- -80 °C
-
- 抗原
- PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))
- 别名
- PARP-1 (PARP1 产品)
- 别名
- ADPRT Protein, ADPRT 1 Protein, ADPRT1 Protein, ARTD1 Protein, PARP Protein, PARP-1 Protein, PPOL Protein, pADPRT-1 Protein, Adprt Protein, Parp-1 Protein, 5830444G22Rik Protein, AI893648 Protein, Adprp Protein, Adprt1 Protein, C80510 Protein, parp-1 Protein, sPARP-1 Protein, BEST:LD21673 Protein, CG17685 Protein, CG17696 Protein, CG17718 Protein, CG40411 Protein, D.PARP Protein, Dm.pARTa Protein, Dmel\\CG40411 Protein, LD21673.3prime Protein, PARP1 Protein, dPARP Protein, parp Protein, adprt1 Protein, padprt-1 Protein, ppol Protein, si:dkey-206f10.3 Protein, wu:fc60f12 Protein, zgc:110092 Protein, poly(ADP-ribose) polymerase 1 Protein, poly (ADP-ribose) polymerase 1 Protein, poly (ADP-ribose) polymerase family, member 1 Protein, Poly-(ADP-ribose) polymerase Protein, poly(ADP-ribose) polymerase 1 L homeolog Protein, PARP1 Protein, Parp1 Protein, Parp Protein, parp1.L Protein, parp1 Protein
- 背景
- The cDNA encoding human poly(ADP-ribose) polymerase (PARP) was cloned by several groups simultaneously. With the discovery of new members (homologs) of the PARP family, PARP is referred to as PARP-1. An isolated cDNA from mouse and human encoded a protein with considerable homology to the catalytic domain of PARP-1. This protein, termed PARP-2, is a 64 kDa protein that contains a nuclear localization signal (NLS) and is activated by DNA breaks, although its DNA- binding domain is very different from that of PARP-1. Evidence has accumulated that PARP plays a role in DNA repair and a substantial effort has been invested to elucidate the physiological function of the PARP pathway in cellular recovery from DNA damage. PARP has been found in the base excision repair (BER) complex with DNA polymerase-, ligase III and x-ray repair cross-complementing 1 (XRCC1). PARP- 1 and PARP-2, even though lacking the zinc- finger domains, bind to single and double strand breaks during oxidative stress. In general, it appears that an early enzymatic activation of PARP occurs upon DNA-strand break formation. Binding of PARP to a DNA nick may then cause a transient halt to cellular activity and protect the DNA from sister chromatid associated proteins such as histones. Nicotinamide is cleaved in this step from the substrate NAD+ by PARP and the so synthesized poly(ADP)-ribose (PAR) is then used to generate ATP.
- 途径
- Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
-
