ERK2 蛋白
Our Local Distributor
北京 101111
Quick Overview for ERK2 蛋白 (ABIN7939320)
抗原
See all ERK2 (MAPK1) 蛋白蛋白类型
宿主
资源
应用范围
-
-
原理
- Recombinant MAPK1 (ERK2) protein
-
-
Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 1-360) protein (GST tag)
MAPK1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Mitogen-Activated Protein Kinase 1 (MAPK1) protein (GST tag,His tag)MAPK1 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 96 % as determined by SDS-PAGE
Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 1-360) protein (His-IF2DI Tag)MAPK1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 1-360) protein (His tag)MAPK1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
限制
- 仅限研究用
-
-
-
缓冲液
- Recombinant MAPK1 (ERK2) protein is supplied at a concentration of 0.3 μg/ul in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.
-
注意事项
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
-
储存条件
- -80 °C
-
储存方法
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
-
-
- ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))
-
别名
- MAPK1
-
背景
-
Protein Background: MAPK1 (ERK2) is a serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating several transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis.
Short Description: Recombinant MAPK1 (ERK2) protein was expressed in a baculovirus expression system as the full length protein (accession number NP_002736.3) with an N-terminal FLAG tag. The molecular weight of MAPK1 is 42.66 kDa.
Aliases: ERk2, Extracellular Signal Regulated Kinase 2, MAP2K, PRKM1, ERK, MAP Kinase 1, ERK-2, ERT1, P40, P41
-
途径
- MAPK Pathway, RTK signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins
抗原
-
